Fig. 3

Overexpression of jmjd-3.2 or utx-1 causes lifespan extension. a, b Levels of jmjd-3.2 (a) and utx-1 mRNA (b) were assessed by qRT-PCR, showing significant upregulation in the jmjd-3.2 and utx-1 transgenic lines, respectively, when either wild-type or demethylase dead (DD) constructs were used. Error bars represent the SEM for each data point. Lifespan assays were performed on transgenic lines overexpressing jmjd-3.2 or utx-1 driven by their respective endogenous promoter. c, d overexpression of either jmjd-3.2 (c) or utx-1 (d) in a wild-type background results in lifespan extension (****p < 0.0001). Overexpression of demethylase dead jmjd-3.2 (jmjd-3.2DD) also results in lifespan extension (****p < 0.0001), whereas overexpression of demethylase dead utx-1 (utx-1DD) has no effect. e Overexpression of either wild-type jmjd-3.2 or demethylase dead jmjd-3.2 (jmjd-3.2DD) does not further extend the lifespan of jmjd-3.2(tm3121) mutants. f Overexpression of wild-type utx-1 in a utx-1(tm3118) mutant background promotes lifespan extension beyond that of the long-lived utx-1(tm3118/+) mutant strain (****p < 0.0001), whereas overexpressing the demethylase dead version has no effect (see Additional file 9: Table S5 for full statistical analysis of lifespan data, including repeats). (OE, overexpression; DD, demethylase dead). N2 control strains contained the same coinjection marker (rol-6⁺) as the jmjd-3.2 and utx-1 transgenic lines