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Table 3 Reported solubility and yield of KRas4B (1–169) from expression in E. coli

From: Assessing optimal: inequalities in codon optimization algorithms

CDS N-terminal taga Solubilityb (%) Yield (mg/L) Reference
Native His6 N.R.e 11 [51]
His8-28mer-His8 41 5 [44]
GeneArt (n = 5) His6 38 23 ± 4 This work
DNA2.0 His6 N.R.e 15 [52]
GeneWiz (opt)c His8-28mer-His8 24 ± 3 15 ± 2 [44]
GeneWiz (KRasRARE)d His8-28mer-His8 52 ± 22 27 ± 11 [44]
  1. aAll N-terminal tags were reported to be followed by a TEV protease cleavage site except the GeneArt sequence that contains a dual Thrombin-TEV cleavage site
  2. bSolubility measurements represent the normalized ratio of overexpressed protein for the total protein and soluble extract lanes observed in SDS-PAGE analysis
  3. cAverage and standard deviation for reported values with 0.1–1.0 mM IPTG
  4. dKRasRARE represents the average and standard deviation for reported values of the GeneWiz optimized sequence w/ either a single or multiple reintroduced rare codons (I46, I84, R164, I84/R164, and I46/I84/R123/R164)
  5. eN.R. not reported
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BMC Biology

ISSN: 1741-7007

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