- Research article
- Open Access
Glucocorticoid-dependent REDD1 expression reduces muscle metabolism to enable adaptation under energetic stress
- Florian A. Britto1,
- Fabienne Cortade1,
- Yassine Belloum1,
- Marine Blaquière2,
- Yann S. Gallot3,
- Aurélie Docquier1,
- Allan F. Pagano1,
- Elodie Jublanc1,
- Nadia Bendridi4,
- Christelle Koechlin-Ramonatxo1,
- Béatrice Chabi1,
- Marc Francaux5,
- François Casas1,
- Damien Freyssenet3,
- Jennifer Rieusset4,
- Sophie Giorgetti-Peraldi6,
- Gilles Carnac2,
- Vincent Ollendorff†1Email author and
- François B. Favier†1Email author
© Favier et al. 2018
- Received: 24 March 2018
- Accepted: 4 May 2018
- Published: 12 June 2018
Skeletal muscle atrophy is a common feature of numerous chronic pathologies and is correlated with patient mortality. The REDD1 protein is currently recognized as a negative regulator of muscle mass through inhibition of the Akt/mTORC1 signaling pathway. REDD1 expression is notably induced following glucocorticoid secretion, which is a component of energy stress responses.
Unexpectedly, we show here that REDD1 instead limits muscle loss during energetic stresses such as hypoxia and fasting by reducing glycogen depletion and AMPK activation. Indeed, we demonstrate that REDD1 is required to decrease O2 and ATP consumption in skeletal muscle via reduction of the extent of mitochondrial-associated endoplasmic reticulum membranes (MAMs), a central hub connecting energy production by mitochondria and anabolic processes. In fact, REDD1 inhibits ATP-demanding processes such as glycogen storage and protein synthesis through disruption of the Akt/Hexokinase II and PRAS40/mTORC1 signaling pathways in MAMs. Our results uncover a new REDD1-dependent mechanism coupling mitochondrial respiration and anabolic processes during hypoxia, fasting, and exercise.
Therefore, REDD1 is a crucial negative regulator of energy expenditure that is necessary for muscle adaptation during energetic stresses. This present study could shed new light on the role of REDD1 in several pathologies associated with energetic metabolism alteration, such as cancer, diabetes, and Parkinson’s disease.
- Skeletal muscle
- Energy expenditure
Regulated in development and DNA damage (REDD1) is a small ubiquitous and conserved protein whose expression is low in basal conditions but highly increased in response to hypoxia, administration of glucocorticoids, endurance exercise, DNA damage, or the unfolding protein response [1–5]. REDD1 is involved in numerous pathologies including oxygen-induced retinopathy , emphysema , Parkinson’s disease , depression , diabetes , and cancer . Many of these diseases are associated with an impairment of skeletal muscle function, i.e., decreased muscle mass and increased fatigability. Muscle function is directly correlated with patient quality of life and mortality [12, 13] and excessive muscle loss reduces the efficacy of pharmacological treatments .
Skeletal muscle function is partially controlled by the Akt/mammalian Target of Rapamycin Complex 1 (mTORC1) pathway. Akt/mTORC1 regulates both skeletal muscle mass and metabolism via activation of several ATP-demanding processes such as protein synthesis , glycogen storage [16, 17], and mitochondrial biogenesis . The Akt/mTOR pathway also controls mitochondrial function by fostering the interaction between mitochondria and the endoplasmic reticulum (ER) in the mitochondria-associated membranes compartment (MAMs) . Indeed, ER transfers Ca2+ to mitochondria , promoting O2 consumption through stimulation of several oxidative enzymes (reviewed in ). Conversely, mitochondria provide ATP to ER for macromolecule biosynthesis, notably proteins . Therefore, activation of the Akt/mTOR pathway leads to an increased O2 uptake [23, 24] and ATP consumption . mTORC1 activation is regulated by Akt-mediated inhibition of PRAS40 and TSC2 [26, 27]. Akt also promotes glycogen storage via mitochondrial association of Hexokinase II (HKII) and inhibition of Glycogen Synthase Kinase 3 (GSK3) [28, 29].
A recognized role for REDD1 is the inhibition of the Akt/mTORC1 pathway through TSC2 activation  or Akt inhibition . Consistently, recent studies have demonstrated that REDD1 is required for mTORC1-dependent protein synthesis inhibition following fasting, sepsis, or dexamethasone (DEX) treatment [32–34]. In addition, REDD1 overexpression is responsible for skeletal muscle atrophy and mTORC1 inhibition [32, 35]. Interestingly, REDD1 expression increases during energetic stresses known to promote amyotrophy such as hypoxia and fasting [36, 37]. Moreover, a pool of REDD1 protein has been localized in the mitochondrial compartment in cultured cells , raising the question of its potential role in mitochondrial respiration or MAMs remodeling. Importantly, recent published data on O2 consumption in REDD1-knockout (KO) mouse embryonic fibroblasts (MEFs) gave contradictory results with either an increase  or a decrease  in basal respiration. However, although recent reviews suggest a metabolic role for REDD1 [40, 41], its physiological role in O2 consumption and adaptation to energetic stress (hypoxia, fasting, and exercise) remains to be elucidated.
We report here that REDD1 expression spares skeletal muscle mass during hypoxia and fasting. We propose that one physiological role of REDD1 is to decrease energetic demand by reducing the mitochondria/ER interaction and by downregulating ATP-dependent synthesis pathways to enable skeletal muscle adaptation during energetic challenges.
REDD1 deletion impairs metabolic adaptation under stress
We previously demonstrated that REDD1 over-expression following glucocorticoid (DEX) treatment or plasmid electroporation promotes skeletal muscle atrophy [32, 35]. Interestingly, REDD1 expression increases in response to hypoxia and fasting [35, 42], two energetic stresses known to promote amyotrophy [36, 37]. We hypothesize that REDD1 could also be responsible for skeletal muscle atrophy during hypoxia and fasting.
To confirm this novel hypothesis further, we challenged REDD1 KO mice with an endurance exercise, another energetic stress condition known to induce REDD1 expression . Once again, REDD1 deletion exacerbated glycogen depletion and AMPK activation in response to exercise (Fig. 1g and h). The higher energetic stress experienced by REDD1 KO mice was also supported by the decrease in aerobic exercise performance compared to WT mice (Fig. 1i). Therefore, REDD1 deletion exacerbates energetic stress leading to skeletal muscle atrophy in hypoxia and fasting and impairment of exercise performance.
REDD1 reduces O2 consumption in skeletal muscle
The exacerbation of energetic stress evidenced in REDD1-depleted conditions could be explained by two non-exclusive hypotheses: (i) a deficiency in ATP production or (ii) an elevation of energy demand. Interestingly, REDD1 has been proposed to localize in the mitochondria in vitro  and recent studies showed that REDD1 deletion in MEFs leads to alteration of mitochondrial function [38, 39]. Therefore, we hypothesized that the increase in energetic stress observed in REDD1 KO mice during metabolic challenges could be due to an alteration of mitochondrial function in skeletal muscle.
In contrast, by performing similar respiration experiments using permeabilized skeletal muscle fibers instead of isolated mitochondria, we observed an increase in VO2 for REDD1 KO compared to WT mice (Fig. 2f). To confirm further that REDD1 directly acts on VO2 in skeletal muscle fibers, we overexpressed REDD1 in WT mice using an AAV. As expected, the intramuscular injection of AAV-REDD1 decreased O2 consumption in WT permeabilized fibers (Fig. 2f) without any change in mitochondrial content (based on citrate synthase activity; Additional file 2: Figure S7). Since no alteration was evidenced with isolated mitochondria, this result suggests that the cellular context is important to reveal the effect of REDD1 on mitochondrial respiration. Collectively, our results indicate that REDD1 may regulate the connection of mitochondria with other adjacent organelles like the ER, rather than directly affecting mitochondrial intrinsic respiration.
REDD1 disrupts the mitochondria/ER interaction
REDD1 inhibits the Akt/mTOR pathway in the mitochondria/ER interface
To assess whether REDD1 could inhibit the Akt/mTOR pathway specifically in MAMs, we analyzed the phosphorylation status of Akt, PRAS40, and mTOR targets (S6 and 4EBP1) in the crude mitochondria fraction (containing MAMs) isolated from fresh muscles. We observed that REDD1 deletion significantly increased PRAS40 phosphorylation (Fig. 4b). As dephosphorylated PRAS40 is known to inhibit mTORC1 by direct binding , we predicted that PRAS40 could also interact with mTOR in this same subcellular compartment. In agreement, endogenous PRAS40 and mTOR co-precipitated in the crude mitochondrial lysate, and this association was strongly increased following REDD1 expression upon DEX treatment in WT but not in REDD1 KO mice (Fig. 4c). Moreover, PRAS40 over-phosphorylation in REDD1 KO mice was associated with a greater phosphorylation of the mTORC1 downstream target S6 in the crude mitochondria fraction (Fig. 4b). This result shows that REDD1 is necessary for inhibiting mTOR activity in MAMs.
To evaluate the impact on protein translation, we monitored puromycin incorporation in human myoblasts under energetic challenging conditions (1 h glucose depletion and ATP synthase inhibition with oligomycin). In these conditions, REDD1 silencing led to an increase in protein synthesis (Fig. 4d). This negative effect of REDD1 on translation appears, however, blunted in the presence of glucose, likely because cells rely mainly on glycolysis rather than on mitochondria to produce ATP (Additional file 2: Figure S9).
mTOR activity has been correlated with energy expenditure measured by basal VO2  or ATP depletion  and we confirmed here that inhibition of mTOR catalytic activity by Torin1 treatment reduced basal VO2 in myoblasts (Additional file 2: Figure S10a). Therefore, REDD1 contributes to reducing VO2 by regulating an Akt/PRAS40/mTOR signaling pathway at the mitochondrial/ER interface and decreasing protein synthesis. The increase in energetic stress during metabolic challenges in REDD1 KO mice could be explained by an inappropriate activation of the Akt/mTOR pathway consistently with the higher activation of the Akt/mTOR pathway found in REDD1 KO mice following exercise or fasting (Additional file 2: Figure S4a, b).
REDD1 limits HKII mitochondrial recruitment
REDD1 reduces basal metabolism in vivo
We have previously shown that REDD1 is important in mediating muscle atrophy caused by chronic glucocorticoid administration . Unexpectedly, we observed in this report that under physiological energetic stress (hypoxia, fasting, or exercise), REDD1’s function in mice is to spare muscle mass and to reduce energy expenditure. This led us to explore the role played by REDD1 in skeletal muscle during these energetically challenging conditions.
REDD1 reduces energetic metabolism and MAMs extent
Our results unveil a metabolic role of REDD1 as a gatekeeper of Akt/mTORC1-mediated synthesis processes leading to a decrease in O2 and ATP consumption. In agreement with data obtained by the group of Lafarge et al. for primary REDD1 KO MEFs , we observed higher whole-body O2 consumption in REDD1 KO mice. Consistently, we showed that REDD1 overexpression reduces O2 consumption in permeabilized fibers prepared from fresh muscles. Interestingly, we did not observe any effect due to REDD1 overexpression or deletion using isolated mitochondria, suggesting that REDD1 could modulate the mitochondrial crosstalk with other cellular organelles or structures such as MAMs, rather than playing a direct role in mitochondrial activity per se. Indeed, we determined here that REDD1 localizes in the MAMs fraction (and not in pure mitochondria) isolated from the skeletal muscles of mice treated with glucocorticoids. Importantly, we demonstrate that REDD1 inhibition resulted in a greater MAMs extent, in cultured human myoblasts and in mice skeletal muscles. Since MAMs promote mitochondrial respiration , our results strongly suggest that REDD1 decreases basal metabolism through inhibition of MAMs formation in response to glucocorticoids, a major component of energetic stress adaptation.
Moreover, we found from a mass spectrometry analysis that endogenous REDD1 and GRP75, a MAM protein bridging ER and the outer mitochondrial membrane, can be co-immunoprecipitated in mice skeletal muscle lysates (data not shown). This suggests that REDD1 may also affect MAMs formation via direct GRP75 binding. Further work is needed to describe fully how REDD1 reduces the extent of MAMs and to identify the molecular mechanisms.
Is REDD1 required for autophagy or mitophagy?
Conflicting results on mitochondrial respiration in REDD1-depleted MEFs have been recently published. On one hand, Lafarge et al. show that REDD1-depleted MEFs had an increased respiration rate, as we observed here for permeabilized myofibers . On the other hand, Qiao et al. reported the reverse outcome in which REDD1-depleted immortalized MEFs exhibit decreased O2 consumption. These authors proposed that REDD1 inhibition impairs mitophagy leading to an accumulation of defective mitochondria . However, we did not find any marker of either defective mitochondria or mitochondrial accumulation in muscles of REDD1 KO mice (citrate synthase and cytochrome c oxidase activities, mitochondrial DNA content, and respiration of isolated mitochondria were all unchanged). Our results indicate that REDD1 appears dispensable for autophagy and mitophagy in skeletal muscle after intensive endurance exercise (assessed by mitochondrial DNA content, and BNIP3 and ULK S317 levels; Additional file 2: Figure S11) as well as after hypoxia (BNIP3, Gabarapl and LAMP2A mRNA expression; Additional file 2: Figure S2). Moreover, it has recently been shown that stimulating OXPHOS enhances mitophagy to facilitate mitochondrial renewal . Incidentally, the raised mitochondrial respiration we observed in REDD1 KO myofibers further supports the higher mitophagy observed in REDD1 KO mice. However, a recent study shows that REDD1 deletion in vivo impairs mitophagy in mice cartilage , suggesting that REDD1’s function could be tissue dependent. Future experiments should help to clarify the role played by REDD1 in autophagy and mitophagy in different cellular contexts.
REDD1 deletion exacerbates energetic stress
We highlight here that the increase in energy and O2 demand in REDD1 KO mice results in a maladaptation to energetic stresses. In fact, upon various energetic challenges (hypoxia, fasting, and exercise), we observed an over-activation of AMPK in the absence of REDD1. Our data agree with a recent study showing an increase in AMPK phosphorylation and ATP depletion in REDD1 KO primary chondrocytes during energetic stress . Parallel to AMPK over-activation, we found greater glycogen depletion after a running exercise or fasting in REDD1 KO compared to WT muscles. This suggests that the fuel requirement is exacerbated in REDD1 KO mice to cope with stress (food deprivation, O2 reduction, or increase in ATP demand). Several studies have shown that activation of Akt increases glycogen content, energy expenditure, and basal VO2 in mice [18, 60, 61]. Glycogen storage depends upon glucose-6-phosphate availability and Akt controls the formation of glucose-6-phosphate, by regulating the mitochondrial localization and activation of HKII . Our results indicate that REDD1 interferes with this cascade of events by inhibiting HKII targeting to mitochondria, therefore limiting glycogen storage and energy demand. In fact, we recorded a higher basal glycogen content in REDD1 KO mice. Thus, in a fed state, REDD1 deletion fosters glycogen storage through over-activation of the Akt/HKII pathway. In contrast, during energetic stress, REDD1 deletion leads to an inappropriate activation of anabolic processes, resulting in increased energy demand and glycogen depletion.
Interestingly, Reiling and Hafen showed that REDD1 overexpression contributed to extend the life span of Drosophila under starvation conditions , underlining the crucial role of REDD1 in adaptation to nutrient deprivation. Moreover, we observed here that REDD1 KO mice display impaired aerobic performance, further demonstrating that REDD1 deficiency disrupts energetic metabolism during exercise. We verified that the hypermetabolic phenotype of REDD1 KO mice (greater O2 consumption and rectal temperature) was not due to a difference in food intake, confirming data reported by others . Chronic elevated energy expenditure leads to a metabolic imbalance that would ultimately result in reduced storage of macronutrients, as we observed in 12-month-old REDD1 KO mice. Altogether, these data reinforce that REDD1’s function is indispensable for skeletal muscle adaptation to energetic stresses and they raise the issue of the role of REDD1 in energetic metabolism homeostasis during aging.
Regulation of muscle mass by REDD1
We previously demonstrated that REDD1 deficiency is protective against skeletal muscle atrophy following glucocorticoid treatment . In contrast, we show here that REDD1 deletion rather exacerbates hypoxia-mediated and fasting-mediated amyotrophy. A likely explanation of this apparent paradoxical effect of REDD1 is that fuel substrates for ATP and macromolecule synthesis are not limiting during glucocorticoid treatment. In this case, sustained Akt/mTORC1-dependent anabolism with REDD1 deletion has no detrimental effects on energetic status and muscle mass. In contrast, during energetic stress (hypoxia and fasting), the limited down-regulation of the Akt/mTORC1 pathway due to the absence of REDD1 will disturb the energy balance (expenditure vs. production). This worsens the metabolic crisis, ultimately resulting in exacerbated muscle wasting. In agreement, we recorded AMPK overactivation in REDD1 KO muscle during energetic stress and AMPK is known to promote muscle protein breakdown through the FOXO-dependent atrophying program . Consistently, we observed increased expression of genes involved in the ubiquitin-proteasome system or in autophagy in hypoxic REDD1-deficient mice.
We propose that REDD1 acts as a rheostat of AMPK activation by limiting an energetic crisis and lowering the energy expenditure caused by unrestrained Akt/mTORC1 signaling, which ultimately restricts the loss of muscle mass. Therefore, trying to maintain the Akt/mTOR-dependent protein synthesis during an amyotrophic process that is secondary to an alteration of energy homeostasis does not appear to be an appropriate therapeutic strategy to spare skeletal muscle mass.
In summary, we highlight a novel physiological metabolic role for REDD1 in glucocorticoid signaling, which enables the acclimatization of skeletal muscle during energetic challenges via the reduction of O2 and ATP consumption assigned to synthesis processes (Fig. 7). This could help our understanding of the pathologies involving REDD1, including cancer, diabetes, emphysema, depression, and Parkinson’s disease.
Whole-body REDD1 null mice were generated by Lexicon Genetics Inc. (The Woodlands, TX) specifically for Quark Pharmaceuticals Inc. (Fremont, CA) as previously described. They are the property of Quark Pharmaceuticals Inc. WT and KO mice were generated from C57Bl6 heterozygous × heterozygous crosses. Genotyping was performed from tail-derived genomic DNA as previously described . Mice were housed in standard cages with free access to food and water under a 12-h dark–light cycle. All animals were killed by cervical dislocation and the tissues were harvested in the morning at the same time each day. These experiments were performed according to European directives (86/609/CEE) and approved by the national ethics committee (referral file APAFIS#9706-2,017,042,516,166,776 v3).
WT and REDD1 KO mice were housed in a hypobaric chamber to simulate an altitude of 6500 m for 11 days after 3 days of progressive acclimatization. Tissues were harvested after cervical dislocation.
WT and KO mice were treated with 1 mg/kg of DEX by oral gavage. Animals were sacrificed 5 h after gavage and their gastrocnemius and quadriceps muscles were removed for subsequent analyses.
Maximum aerobic velocity was determined during a running exercise using a motorized treadmill. The speed was set to 10 m/min for 2 min, after which it was increased by 1 m/min every 60 s until exhaustion. An intensive exercise experiment was performed by setting the speed to 10 m/min for 60 min, after which it was increased by 1 m/min every 2 min for 30 min. Muscles were removed immediately at the end of the experiment period (90 min) after cervical dislocation and they were quickly frozen in liquid nitrogen and stored at −80 °C. Before each experiment (determination of maximum aerobic velocity or prolonged exercise), the mice were accustomed to the treadmill with a 5-min run at 10 m/min for 3 d, the last session occurring 48 h before the test.
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blots were performed as previously described . Briefly, all antibodies were incubated on membranes overnight at 1:1000 except for α-tubulin (1:2000), mTOR, HK II, calreticulin, histone H3, ULK1, and phospho-ULK1 ser757 and ser317 (1:500) and IP3R (1:125). The next day, membranes were washed and incubated with HRP (Horseradish peroxidase)-conjugated secondary antibody at 1:3000 for 1 h at room temperature. Membranes were developed with Enhanced chemioluminescent (ECL) reagent using a Chemidoc™ Touch Imaging System (BioRad). A list of all antibodies is given in Additional file 1: Table S2.
Skeletal muscle subcellular fractionation
Subcellular fractionation was performed on fresh muscles according to the method described by Dimauro et al. . Briefly, the right and left gastrocnemius muscles were homogenized in STM buffer (250 mM sucrose, 50 mM Tris HCl (pH 7.4), 5 mM MgCl2, and a protease/phosphatase inhibitor cocktail). Muscle homogenate was centrifuged at 800g to pellet the nuclear fraction. The nuclear fraction was successively washed and centrifuged three times in STM buffer, then resuspended in NET buffer [20 mM HEPES (pH 7.9), 1.5 mM MgCl2, 0.5 M NaCl, 0.2 mM ethylenediaminetetraacetic acid (EDTA), 20% glycerol, 1% triton X100, and a protease/phosphatase inhibitor cocktail]. Other fractions were centrifuged at 800g to eliminate potentially contaminating nuclei still present in the homogenate. Then, the supernatant was centrifuged at 11,000g to separate the crude mitochondrial fraction (pellet) from the cytosolic and microsomal fraction (supernatant). The mitochondrial fraction was washed in STM buffer and centrifuged at 11,000g and finally resuspended in SOL buffer [50 mM Tris-HCl (pH 6.8), 1 mM EDTA, 0.5% triton X100, and a protease/phosphatase inhibitor cocktail] or CHAPS buffer for immunoprecipitation (0.3% CHAPS, 40 mM HEPES pH 7.5, 120 mM NaCl, 1 mM EDTA, 50 mM NaF, 1.5 mM Na3VO4, 10 mM β-glycerophosphate, and a protease inhibitor cocktail). The cytosolic and microsomal fraction was ultra-centrifuged at 100,000g to pellet the microsomes and other contaminant organelles. Cytosolic proteins in the supernatant were precipitated with acetone and centrifuged at 12,000g. Finally, the supernatant was discarded and the cytosolic proteins precipitated in the pellet were resuspended in STM buffer. MAMs fractions were isolated from the crude mitochondrial fraction as previously described .
Immunoprecipitation of mitochondrial fraction
Gastrocnemius mitochondria were obtained by subcellular fractionation as explained above. In total, 500 μg of proteins from the lysate were diluted in a final volume of 500 μl with the CHAPS buffer. Precleared lysates were incubated with either 3 μg of anti-PRAS40 or 3 μg of anti-rabbit immunoglobulin G overnight at 4 °C. Samples were then incubated 1 h at 4 °C with 100 μl of 20% protein G sepharose beads (Cell Signaling Technology), collected by centrifugation, and washed four times with CHAPS buffer before analysis by western blot.
Gastrocnemius muscles were quickly excised and immediately placed into ice-cold buffer (100 mM KCl, 5 mM MgSO4, 5 mM EDTA, and 50 mM Tris-HCl, pH 7.4). Muscles were freed of connective tissues, minced, homogenized with an Ultra-turax homogenizer, and treated with Subtilisin A (0.1 mg/g wet muscle). Mitochondria were separated by centrifugation at 8000g, then at 800g. Finally, the mitochondria were pelleted from the supernatant at 9000g. The mitochondria were resuspended in 100 mM KCl and 10 mM MOPS (3-(N-Morpholino) propanesulfonic acid), pH 7.4. Mitochondria oxygen consumption was measured using the high-resolution Oxygraph-2 k (OROBOROS Instruments). Isolated mitochondria were incubated in two sealed thermostated chambers (37 °C) containing 2 ml of MIRO5 respiration medium (0.5 mM EGTA, 3 mM MgCl2.6H2O, 65 mM KCl, 20 mM taurine, 10 mM KH2PO4, 20 mM HEPES, 110 mM sucrose, and 1 g/l bovine serum albumin, pH 7.1). Resting rate (state 4) was evaluated in the presence of 2.5 mM malate, 5 mM glutamate, and 5 mM succinate or 40 μM palmitoylcarnitine and 2.5 mM malate. ADP-stimulated rate (state 3) was determined after addition of 0.5 mM ADP. Oxygen consumption was normalized to the total protein content of each sample measured using the Bradford assay.
For permeabilized fibers experiment, about ten pools of four muscle fibers were isolated from the tibialis anterior in ice-cold biopsy preservation solution (BIOPS: K2-EGTA 7.23 mM, CaK2-EGTA 2.77 mM, imidazole 20 mM, dithiothreitol 0.5 mM, KH2PO4 3 mM, MgCl2 6.56 mM, bovine serum albumin 0.2%, taurine 20 mM, ATP 5.3 mM, 2-(N-morpholino) ethane-sulfonic acid hydrate 53.3 mM, phosphocreatine 15 mM, pH 7.1). Fibers were then transferred into 1 ml of BIOPS containing 50 μg/ml of saponin for 30 min and washed twice in BIOPS free from saponin. The permeabilized fibers were introduced into an oxygraph chamber containing MIRO5 medium. Respiration was recorded after addition of the following substrates: pyruvate 5 mM, malate 5 mM, and ADP 5 mM. Oxygen consumption was normalized to the total protein content of each sample measured using the Bradford assay. Data acquisition and analysis were performed using Oxygraph-2 k-DatLab software version 4.3. The respiratory control ratio was calculated as the ratio of oxygen consumption at state 3 over oxygen consumption at state 4.
Respiratory gas exchanges were measured at 22 °C using a Comprehensive Lab Animal Monitoring System (Columbus Instruments) as previously described . Mice were acclimatized individually in metabolic cages for 16 h before the 24 h recording. The respiratory exchange ratio was calculated as the ratio between CO2 production and O2 consumption.
Muscle glycogen content was measured as previously described . Briefly, 30–50 mg of quadriceps muscle was dissolved in 30% KOH saturated with Na2SO4 at 100 °C for 20 min. Glycogen was precipitated by addition of 1.2 vol of 95% ethanol and the sample was centrifuged at 840g (20 °C) for 20 min. The glycogen precipitate was dissolved in 300 μl distilled water and diluted at 1/10. Then, 200 μl of 5% phenol solution and 1 ml of H2SO4 were added to 200 μl of the glycogen solution obtained, which was then incubated at room temperature for 10 min and at 27 °C for 15 min. Glycogen concentration was determined spectrophotometrically at 490 nm.
Mitochondrial DNA measurement
Total DNA or RNA was extracted from the gastrocnemius muscle using a QIAamp DNA or RNA mini kit (Qiagen). Genomic (ATP synthase beta) and mitochondrial DNA (NADH dehydrogenase subunit 5) were quantified by quantitative real-time polymerase chain reaction (qPCR; primer sequences are listed in Additional file 1: Table S3). qPCR was performed using KAPA 2× SYBR green master mix on an Miniopticon thermocycler. Cycling conditions were 1 cycle at 98 °C for 30 s following by 40 cycles at 95 °C for 1 s and 60 °C for 15 s. The fusion index was measured for increments of 0.5 °C every 5 s (starting at 65 °C and finishing at 95 °C). Each sample was run in duplicate. Results were expressed using the comparative cycle threshold (Ct) with genomic DNA as the control. The relative changes in the expression level of mitochondrial DNA were calculated by the ΔΔCt formula.
Firstly, 10 ng of each complementary DNA (cDNA) was initially pre-amplified with Taqman PreAmp Master Mix (Applied Biosystems) and a pool containing all the primers (Additional file 1: Table S3) targeting all the genes (200 nM each). To prepare samples for loading into the IFC, a mix was prepared consisting of 440 μl 2X TaqMan Master Mix (Applied 430,976), 44 μl 20× DNA Binding Dye Sample Loading Reagent (Fluidigm 100-3738), 44 μl 20X Evagreen (Biotium 31,000) and 132 μl TE (Tris10mM, EDTA 1mM). Then, 6 μl of this mix was dispensed into each well of a 96-well assay plate. Next, 2 μl of the pre-amplified cDNA sample was added to each well and the plate was briefly vortexed and centrifuged. Following priming of the IFC (Integrated Fluidic Circuit) in the IFC Controller HX, 5 μl of the cDNA sample plus reagent mix were dispensed to each sample inlet of the 96.96 IFC. For the assays, 5 μl of each 10X Assay (5 μM of each primer) were dispensed to each detector inlet of the 96.96 IFC. After loading the assays and samples into the IFC in the IFC Controller HX, the IFC was transferred to the BioMark and PCR was performed using the following thermal protocol: thermal mix at 50 °C for 2 min, 70 °C for 30 min, 25 °C for 10 min, hot start at 50 °C for 2 min, 95 °C for 10 min, 35 cycles of PCR (95 °C for 15 s and 60 °C for 60 s), and melting analysis. The results were analyzed using the Fluidigm real-time PCR analysis software v.4.1.2 and expressed using the comparative cycle threshold (Ct) with tubulin mRNA expression as the control.
Primary culture of human myoblasts
A quadriceps muscle biopsy from one healthy male adult (30 years old) was taken at the Centre Hospitalier Universitaire Lapeyronie (Montpellier, France). The volunteer signed informed written consent after being described the protocol. Myoblasts were purified from muscle biopsies and were grown on collagen-coated dishes in Dulbecco’s modified Eagle’s medium/F12 with 10% fetal bovine serum (FBS), 0.1% Ultroser G and 1 ng/ml of human basic FGF (Fibroblast Growth Factor) (growth medium) as previously described . For transient siRNA transfections, REDD1 (siREDD1) and negative control (siCTRL) Silencer Select pre-designed siRNAs were purchased from Life Technologies (France). Human myoblasts were transfected with siREDD1 (#S29168, Ambion) and siCTRL as previously described  and harvested 48–72 h after transfection. The mean inhibition was 81.4 ± 2.3%. ATP content was quantified by CellTiter-Glo Luminescent Cell Viability/ATP Assay kit (Promega), and normalized by DNA content. For protein synthesis labelling, puromycin (Sigma-Aldrich) was diluted in cell culture media (final concentration 5 μg/ml) for 30 min. Western blots with anti-puromycin antibody (1:2000) were normalized using the Biorad stain-free method.
Duo-link proximity ligation in situ assay
Cells were fixed 30 min at room temperature with 4% formaldehyde and permeabilized for 10 min at room temperature with phosphate-buffered saline 0.1% Triton X100. PLA was performed according to the Duolink II in situ PLA kit (Sigma-Aldrich) protocol. Nuclei were stained with Hoechst and fluorescence was analyzed with a fluorescence ApoTome microscope (Zeiss). We used the Blobfinder software (Centre for Image Analysis, Uppsala University) to quantify the spot/nucleus signal. For skeletal muscle, in situ PLA was performed on paraffin-embedded sections of gastrocnemius using a bright-field revelation. The nuclei in longitudinal sections were stained and IP3R/VDAC spots were quantified at 20× magnification (ten fields per muscle).
Results are presented as mean ± standard error of mean. All data were tested for normality through skewness and kurtosis or homoscedasticity analyses and then analyzed by Student’s t-test or two-way ANOVA (with genotype and stress condition as the two factors). A Fisher's least significant difference post hoc analysis was used to determine differences between groups when ANOVA was significant. Statistical significance was set to p < 0.05.
We are grateful to Quark Pharmaceuticals for providing the REDD1-KO mice. We thank the animal staff from the METAMUS DMEM platform facility, which belongs to the "Montpellier animal facilities network" (RAM). We thank Clémence Genthon and the GenoToul platform (Toulouse, France) for mRNA analyses. We also acknowledge the MRI imaging facility, which is a part of the national bioimaging infrastructure supported by the French National Research Agency (ANR-10-INBS-04, Investments for the future). Mass spectrometry experiments were carried out using the facilities of the Functional Proteomics Platform of Montpellier.
This work was supported by the "Agence Française de Lutte contre le Dopage" (grant 4299).
Availability of data and materials
All data generated during this study are included in Additional file 3.
FAB, VO, and FBF conceived of and designed the research. FAB, FCo, YB, MB, YSG, AD, AFP, EJ, NB, CR, FCa, JR, GC, VO, and FBF performed the experiments. BC, MF, DF, FCa, JR, SGP, and GC provided the key reagents and gave experimental advice. FAB, VO, and FBF analyzed the data and interpreted the results of the experiments. FAB and FBF prepared the figures. FAB, VO, and FBF drafted the manuscript. All authors read and approved the final manuscript.
Ethics approval and consent to participate
All procedures on mice comply with European directives (86/609/CEE) and were approved by the national ethics committee (referral file APAFIS#9706-2017042516166776 v3).
The authors declare that they have no competing interests.
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