- Research article
- Open Access
Functional role of dimerization and CP190 interacting domains of CTCF protein in Drosophila melanogaster
- Artem Bonchuk†1,
- Oksana Maksimenko†2,
- Olga Kyrchanova†1,
- Tatyana Ivlieva1,
- Vladic Mogila1, 4,
- Girish Deshpande3,
- Daniel Wolle3,
- Paul Schedl2, 3Email author and
- Pavel Georgiev1Email author
© Bonchuk et al. 2015
Received: 15 May 2015
Accepted: 15 July 2015
Published: 7 August 2015
Insulators play a central role in gene regulation, chromosomal architecture and genome function in higher eukaryotes. To learn more about how insulators carry out their diverse functions, we have begun an analysis of the Drosophila CTCF (dCTCF). CTCF is one of the few insulator proteins known to be conserved from flies to man.
In the studies reported here we have focused on the identification and characterization of two dCTCF protein interaction modules. The first mediates dCTCF multimerization, while the second mediates dCTCF–CP190 interactions. The multimerization domain maps in the N-terminus of the dCTCF protein and likely mediates the formation of tetrameric complexes. The CP190 interaction module encompasses a sequence ~200 amino acids long that spans the C-terminal and mediates interactions with the N-terminal BTB domain of the CP190 protein. Transgene rescue experiments showed that a dCTCF protein lacking sequences critical for CP190 interactions was almost as effective as wild type in rescuing the phenotypic effects of a dCTCF null allele. The mutation did, however, affect CP190 recruitment to specific Drosophila insulator elements and had a modest effect on dCTCF chromatin association. A protein lacking the N-terminal dCTCF multimerization domain incompletely rescued the zygotic and maternal effect lethality of the null and did not rescue the defects in Abd-B regulation evident in surviving adult dCTCF mutant flies. Finally, we show that elimination of maternally contributed dCTCF at the onset of embryogenesis has quite different effects on development and Abd-B regulation than is observed when the homozygous mutant animals develop in the presence of maternally derived dCTCF activity.
Our results indicate that dCTCF–CP190 interactions are less critical for the in vivo functions of the dCTCF protein than the N-terminal dCTCF–dCTCF interaction domain. We also show that the phenotypic consequences of dCTCF mutations differ depending upon when and how dCTCF activity is lost.
The chromatin fiber in the chromosomes of multicellular animals is organized into a hierarchical set of topologically independent domains [1–6]. This hierarchical loop domain organization is critical for the proper utilization and propagation of the genetic information encoded by the chromosome and it is intimately involved in such processes as gene regulation, replication, recombination, repair, and mitosis. A special set of architectural elements, called boundaries or insulators, are responsible for both subdividing the chromatin fiber into discrete domains and determining their hierarchical organization. These architectural elements have a seemingly contradictory set of functions. When placed between enhancers/silencers and their target genes, they block regulatory interactions [1, 7–11]. However, insulators also have the ability to promote regulatory interactions between enhancers/silencers and their target genes. One mechanism depends upon insulator pairing interactions that connect distant chromosomal segments together at the base of a topological loop [12, 13]. When the regulatory elements in the loop are properly oriented, these insulator–insulator pairing interactions can place distant enhancers/silencers in close proximity to target genes. Additionally, there is suggestive evidence that insulators can have an even more direct role in mediating enhancer–promoter interactions [14–21].
Nearly a dozen DNA binding proteins that have insulator/architectural activity have been discovered. Of these, the most broadly conserved protein is the CCCTC-binding factor, CTCF . Except for a few lineages that have apparently lost the CTCF gene, it is a characteristic feature of bilaterian organisms [23–25]. CTCF was initially identified as a transcription factor that can both repress and activate transcription [26, 27]. Subsequent experiments in flies and vertebrates showed that CTCF also has enhancer-blocking activity and to date it is the only mammalian protein has been shown to have this insulator activity [28–30]. In addition to its roles in gene regulation, a growing body of evidence points to an architectural function. For example, in vertebrates CTCF mediates specific long-distance interactions between insulators and regulatory elements located at megabase distances and even on different chromosomes [31–34]. Similarly, Drosophila dCTCF can support pairing-dependent insulator bypass in transgene assays when two sets of multimerized binding sites for the protein are arranged in the appropriate orientation [15, 35].
Like the CTCF proteins of vertebrates, Drosophila CTCF contains 11 C2H2 zinc fingers flanked by N-terminal domains (NTDs) and C-terminal domains (CTDs) . The dCTCF zinc fingers show significant homology with their vertebrate counterparts, and this is reflected in the similar sequence recognition properties of the vertebrate and fly proteins . In contrast, the NTDs and CTDs are not well conserved and there are reasons to think that the differences between the NTDs and CTDs of vertebrate and fly proteins have mechanistic implications. The insulator/architectural activities of vertebrate CTCF depend at least in part upon its ability to recruit the cohesin complex to specific chromosomal sites [38–41]. Cohesin knockdowns were shown to impair both the insulator (enhancer-blocking) and architectural (long-distance interactions) functions of CTCF [31, 42, 43]. The vertebrate cohesin complex consists of four proteins: Smc1, Smc3, Scc1, and SA/STAG . Smc1 and Smc3 form a ring in the presence of ATP, and this ring is stabilized by the binding of Scc1 and SA/STAG. Vertebrate CTCF is thought to recruit cohesins to specific chromosomal sites by interacting directly with the SA/STAG subunit of cohesin complex. Xiao et al.  have shown that sequences in the C-terminal tail of CTCF are responsible for specific interactions with SA/STAG, and that insulator activity and cohesin recruitment are disrupted when this region of the CTCF protein is mutated.
dCTCF differs from its vertebrate counterpart in that it does not appear to co-localize with cohesins [44, 46]. This finding has led to the idea that other proteins might fulfill the long-distance linking function envisioned for cohesins. One plausible candidate is CP190 . It was originally identified as a microtubule binding protein that associates with the centrosome during mitosis [48, 49]. However, subsequent studies argued against a centrosome or mitotic function and instead pointed to a role in some aspect of nuclear architecture or chromosome structure [50, 51]. Support for this idea came from the discovery that CP190 is required for the enhancer-blocking activity of the gypsy Su(Hw) insulator and is a component both of this transposon insulator and of endogenous Su(Hw) insulators [52, 53]. The connection to chromosome architecture was further supported by studies showing that CP190 localizes to many dCTCF sites and can be co-immunoprecipitated with dCTCF [54–57].
In the studies reported here we have examined the functioning dCTCF protein in more detail. Using a combination of biochemical and genetic approaches we have identified the CP190 interaction domain. We also uncovered a dCTCF dimerization/multimerization domain that, like CP190, could potentially mediate interactions between distant DNA sequences containing dCTCF in vivo. In the course of this analysis we have re-examined the effects of dCTCF null mutations, and tested whether dCTCF proteins lacking the CP190 interaction domain or the dCTCF dimerization/multimerization domain can rescue the null mutation.
dCTCF contains an N-terminal dimerization domain
One of the interactions that could be mediated by these modules was the dimerization or multimerization of the dCTCF protein. This possibility was suggested by insulator bypass experiments in which pairs of multimerized and appropriately oriented CTCF binding sites could mediate long-distance regulatory interactions [15, 16]. Additionally, studies on vertebrate CTCF have suggested that it can form dimers [58, 59]. To test for the presence of homo-dimerization/multimerization modules in the N- or C-terminus of the dCTCF protein, we fractionated bacterially expressed thioredoxin-fused NTD or CTD proteins by size-exclusion chromatography. As shown in Fig. 1b, the thioredoxin NTD fusion 1–288 had a hydrodynamic molecular mass significantly larger (~250 kD) than that predicted for the monomer (45 kD). Similar results were obtained for the CTD 612–818 protein (Fig. 1b).
The presence of these larger complexes could be explained by either a module-dependent multimer formation or by the presence of intrinsically disordered regions that lead to non-specific protein aggregation. To distinguish between these possibilities we used glutaraldehyde cross-linking to probe for complex formation. In the case of the CTD 612–818 protein, glutaraldehyde cross-linking was quite inefficient, suggesting that it likely forms non-specific aggregates (Additional file 2: Figure S2A). On the other hand, consistent with the results of the size-exclusion chromatography, cross-linking of the NTD 1–288 protein gave a high yield of a multimeric band of ~200 kD (Fig. 1c).
To further pinpoint the interaction module, we generated three C-terminal deletions (see Fig. 1d). The smallest deletion, NTD 1–205, gave a cross-linked band of ~100 kD. The largest C-terminal deletion, NTD 1–125, also gave a cross-linked product; however, the yield was quite low compared to the NTD 1–205 protein (compare Additional file 2: Figure S2A to Fig. 1c). This suggests that key interaction sequences were located between amino acids 125 and 205. This suggestion was supported by NTD 1–163, which was much more efficiently cross-linked than NTD 1–125 (Fig. 1c). Though NTD 1–163 gave a prominent cross-linked band at the approximate size expected for the tetramer (~120 kD), there was also a ladder of larger bands. This ladder was likely due, at least in part, to the presence of the thioredoxin moiety in the fusion protein, as only two cross-linked bands were observed when thioredoxin was removed (Additional file 2: Figure S2A). Taken together, these findings map the N-terminal dCTCF:dCTCF multimerization module to sequences spanning the region between amino acids 125 and 163 and suggest that this module likely mediates the formation of dimers or possibly tetrameric complexes. Further support for the formation of multimeric complexes (tetrameric or an even larger) came from size-exclusion chromatography of the NTD 1–163 protein (lacking the thioredoxin moiety), which gave a predicted mass of 120 kD (Fig. 1e). However, it is also possible that disordered regions of the protein retard complex mobility during size-exclusion chromatography.
Several additional lines of evidence localized the dCTCF multimerization module to this region of the NTD. First, two internal deletions (Fig. 1d) that lacked sequences from this interval failed to cross-link efficiently (Additional file 2: Figure S2A). Second, two terminally truncated proteins, NTD 125–180 and NTD 70–163 (Fig. 1d), that contained this part of the NTD were cross-linked efficiently (Additional file 2: Figure S2A). Third, protease digestion indicated that the region containing the interaction module had an ordered structure. We subjected the thioredoxin NTD 1–205 fusion protein to limited proteinase K or trypsin digestion and then analyzed the resulting protease-resistant products by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (Additional file 2: Figure S2B; Additional file 3: Table S1). Both proteases generated two resistant-to-digestion products. One corresponded to thioredoxin, while the other to a dCTCF NTD peptide extending from 84 to 188.
To independently demonstrate that the NTD contains a dCTCF multimerization module we used two different in vivo assays. The first was a yeast two-hybrid assay (Fig. 1f). Sequences encoding the NTD 1–288 amino acids were fused in-frame to the yeast GAL4 DNA binding domain (BD) and activation domain (AD). Because steric hindrance can interfere with transcriptional activation in the two-hybrid system, the NTD 1–288 sequence was placed at both the N-terminus (NTD-AD and NTD-BD) and the C-terminus (AD-NTD and BD-NTD) of the fusion protein. Fig. 1f shows that activation was observed in only one configuration, NTD-BD and AD-NTD. Similar results were obtained when the NTD was tested with a full-length dCTCF protein (not shown).
The BTBCP190 dimer interacts with the C-terminal domain of dCTCF
To identify modules in dCTCF that mediate CP190 interaction, we first used the yeast two-hybrid assay. dCTCF was subdivided into the NTD, the zinc fingers plus the CTD, and the CTD alone and each was fused to the GAL4 activation domain. The NTD failed to interact, while the full-length protein, the zinc fingers plus the CTD, and the CTD alone gave transcriptional activation when combined with full-length CP190 (Fig. 3b). The localization of the CP190 interaction module in dCTCF to the CTD was confirmed by GST pull-down experiments. A GST-fusion protein containing the dCTCF-CTD 612–818 domain was found to pull down both bacterially expressed CP190 and CP190 in Drosophila S2 cell nuclear extracts (Fig. 3c,d).
To further pinpoint the sequences within the 612–818 CTD that are important for contacting CP190, we expressed two different FLAG-tagged C-terminal deletions, dCTCFΔ610-723 and dCTCFΔ774-818, in Drosophila S2 cells. Figure 3e shows that FLAG-tagged wild-type dCTCF and a control N-terminal deletion could be precipitated by CP190 antibodies from the S2 extracts. In contrast, neither of the smaller dCTCF-CTD deletions was precipitated by CP190 antibodies from S2 cells. Taken together, these findings indicate that an apparently rather large sequence is required to mediate a dCTCF–CP190 association that is stable in S2 nuclear extracts.
We used a similar strategy to localize the region in the CP190 protein that mediates interactions with dCTCF. For the yeast two-hybrid experiments, full-length dCTCF was fused to the GAL4 activation domain, while different sub-fragments from CP190 were fused to the GAL4 DNA BD. These experiments map a dCTCF interaction module to the CP190 BTB domain (Fig. 3b). This was confirmed by GST pull-down experiments (Fig. 3f) which showed strong protein–protein interactions between the CP190 BTB domain (1–126) and dCTCF. In addition, weak interactions were detected between the dCTCF-CTD and GST–CP190 fusions spanning the microtubule interaction domain (see CP190 308–517 and 308–468 in Fig. 3f).
Rescuing a dCTCF null allele
If the NTD and CTD interaction modules are critical for dCTCF function, then dCTCF proteins that lack these modules should be defective. As a prelude to assaying the activity of NTD and CTD mutant proteins in vivo, we determined whether dCTCF mutant flies could be rescued by a transgene expressing the wild-type dCTCF protein. Several putative dCTCF null alleles have been reported [54, 55, 63]. Flies homozygous for these mutations (dCTCF 30.6 , dCTCF Y+1 , dCTCF Y+2 , dCTCF 30 ) mainly died during larvae–pupae stages. For the wild-type rescue construct we generated P-element transformants of a hybrid fusion gene that expresses the dCTCF cDNA under the control of the ubiquitously expressed hsp83 promoter [64, 65]. To identify the transgene proteins, a sequence encoding a 3xFLAG epitope was introduced at the beginning of the dCTCF open reading frame. Five independent transgene inserts were recovered on the first and second chromosomes; however, none of these transgenes rescued the lethal effects of the four dCTCF alleles. One reason why the transgenes were unable to complement the four dCTCF alleles is that they did not express as much protein as the endogenous dCTCF gene. An alternative possibility is that there were additional lethal lesions on the chromosomes carrying these particular dCTCF mutations.
To confirm these findings, we generated two imprecise excisions, GEx52 and GEx56, by introducing the P transposase. As indicated in Additional file 4: Figure S3, both imprecise excisions disrupted the coding sequence and were expected to encode only a truncated protein containing the first ~158 N-terminal amino acids. Both excision derivatives had the same phenotypic effects as GE24185 and were complemented by the hsp83-dCTCF + transgene. These results support the conclusion that GE24185 is a null allele of the dCTCF gene .
As was shown previously , adult flies homozygous for the GE24185 mutation as well as the two excision derivatives had a mild but highly penetrant held out wing phenotype and thin bristles throughout the animal, and exhibited a series of homeotic phenotypes in posterior parasegments indicative of a loss of Abd-B activity. These homeotic phenotypes were temperature dependent. They were typically observed in flies raised at 25 °C, while they were much less frequent when the flies were raised at 18 °C. One of these phenotypes was the presence of a rudimentary A7 segment in males as would be expected for a loss-of-function transformation of PS12 into PS11 (Fig. 5d). Another was a protruding and rotated male genitalia. Also unlike wild-type males, GE24185 males had bristles on the A6 sternite and sometimes also patchy pigmentation of the A5 tergite. The former phenotype is characteristic of a PS11 to PS10 transformation, while the latter is expected for a PS10 to PS9 transformation. While A7 and A8 do not form cuticular structures in adult males, they contribute to the cuticle in females. Homeotic transformations of the A7 sternite into A6 were evident in surviving GE24185 females (Additional file 5: Figure S4). Adult mutant females had significantly reduced egg production, and produced no viable offspring when mated to homozygous mutant males. These lethal effects could, however, be rescued by mating the homozygous mutant females to heterozygous balancer males. This finding indicates that zygotic dCTCF expression can compensate for the absence of maternally derived dCTCF.
Selective depletion of dCTCF from the bithorax complex in GE241845 pupae
Mohan et al. (2007) found that though the levels of dCTCF were substantially reduced in GE24185 larvae, maternally derived protein could still be detected at approximately 25 % of the sites in salivary gland polytene chromosomes that are normally observed in wild-type polytenes . One idea suggested by this observation is that the homeotic transformations evident in GE24185 adults arise because dCTCF is selectively lost from the bithorax complex (BX-C). When dCTCF depletion compromises BX-C insulator function, this might enable Polycomb response elements (PREs) in silenced cis-regulatory domains to repress neighboring active cis-regulatory domains and thus downregulate Abd-B expression in a manner that changes segmental identity. Alternatively, or in fact in addition, the proper functioning of the Abd-B promoter could require dCTCF.
ChIP experiments with chromatin isolated from wild-type pupae using antibodies directed against the N-terminal region of dCTCF confirmed that it was bound to the insulators and the Abd-B promoter in BX-C and to the CG1354 promoter (9A1) and four BGL3 insulators (Fig. 6). However, the extent of enrichment of Fab-3, Fab-4, and the Abd-B promoter was about half that of the other BX-C insulators (Mcp, Fab-6, and Fab-8) and also of the CG1354 promoter (9A1) and four BGL3 insulators. As expected, the enrichment of the BX-C dCTCF sequences was substantially reduced in ChIPs of homozygous GE24185 pupae. The extent of reduction was not, however, uniform. Near-background levels of dCTCF were observed for Fab-3, Fab-4, Fab-8, and the Abd-B promoter in GE24185 mutant pupae, while residual dCTCF could still be detected at Mcp and, to a lesser extent, Fab-6. By contrast, only one BGL3 insulator, 24C4, showed a loss of dCTCF equivalent to that seen for most of the BX-C sequences. Three of the other insulators, 9A1, 21E2, and 57B4R, showed only very modest reductions. Though the loss of dCTCF at the fourth insulator, 27B2, was more substantial, the occupancy level in mutant pupae was still about the same as that seen for several of the BX-C elements in wild type.
The rather modest reductions in dCTCF evident at several non-BX-C insulators as well as the residual dCTCF that was retained at two of the BX-C insulators in the absence of a zygotic source of dCTCF protein would suggest that a significant amount of maternally derived dCTCF remains up to at least the pupal stage in GE24185 mutant animals. Moreover, it would appear that the protein is preferentially retained at a subset of the dCTCF insulators. However, an alternative explanation for the apparent persistence of maternal dCTCF is that our antibody recognized some other protein species that happened to bind to the insulators that were pulled down in ChIPs of the mutant pupae. To exclude this possibility, we used an antibody directed against the C-terminal region of the dCTCF protein for ChIP experiments (Additional file 6: Figure S5). ChIPs with this antibody paralleled those obtained with the N-terminal antibody. Substantial amounts of dCTCF persisted at several non-BX-C insulators in GE24185 mutant pupae, while there was still some residual dCTCF remaining at the BX-C insulators Mcp and Fab-6.
With the exception of Mcp, all of the BX-C insulators and the Abd-B promoter had less CP190 than the BGL3 insulators. The effects of GE24185 mutation on CP190 association with the BX-C and BGL3 sequences also followed a pattern similar to that observed for dCTCF. For all of the BX-C insulators, loss of dCTCF was accompanied by a loss of CP190. For the other insulators, the reduction in CP190 occupancy was, with one exception, roughly comparable to that seen at the insulator for dCTCF. For example, dCTCF levels were reduced about 40 % for 9A1, while CP190 was reduced about 50 %. The one exception was 27B2, which lacked CP190 in GE24185 mutant pupae, yet retained significant dCTCF occupancy. To confirm that the loss of CP190 occupancy at dCTCF insulators was not due to a reduction in CP190 protein levels, we probed western blots of extracts prepared from wild-type and GE24185 mutant pupae (Additional file 7: Figure S6).
Role of CTDs and NTDs in functional activity of the dCTCF protein
To examine the in vivo functions of the N-terminal multimerization domain and the C- terminal CP190 interacting domain, we generated hsp83 transgenic lines expressing FLAGx3-tagged dCTCF proteins lacking these domains. For the multimerization domain, we deleted sequences between amino acid 90 and amino acid 170 (dCTCFΔN). This deletion spans the region required for dCTCF–dCTCF interactions in vitro. For the CP190 interaction module, we used the C-terminal 774–818 deletion (dCTCFΔC) that eliminates interactions between CTCF and CP190 in S2 cells (Fig. 5c). The activities of two independent transgenic lines expressing the deleted proteins were tested in the GE24185 mutant background.
As described above, the control transgene, dCTCF + , encoding the wild-type protein fully complemented the zygotic and maternal effect lethality of the GE24185 mutation (Fig. 5d). It also rescued the thin bristles phenotype and the loss-of-function homeotic transformations evident in PS11-14 (Fig. 5 and Additional file 5: Figure S4). However, in approximately 10 % of the dCTCF + males we observed a partial loss of pigmentation in the tergite of abdominal segment A5, which is characteristic of a loss-of-function transformation of A5 (PS10) to A4 (PS9) transformation . The held out wing phenotype was also not rescued. The dCTCF ΔC transgenes resembled dCTCF + . They fully rescued the zygotic and maternal effect lethality of the GE24185 mutations, the thin bristles, and the PS11-14 homeotic transformations in males and females. Like dCTCF + , we also observed a partial loss of pigmentation on the A5 tergite; however, the frequency was somewhat higher (50 % as compared to 10 %) and the size of the depigmented patches was typically larger. In contrast to dCTCF ΔC , the dCTCF ΔN transgene only partially ameliorated the zygotic and maternal effect lethality of GE24185 and dCTCF ΔN transgenic flies had reduced viability and were only semi-fertile. In addition, dCTCF ΔN did not rescue the thin bristles phenotype or the homeotic transformation seen in the abdominal segments of GE23185 adult males and females (Fig. 5d and Additional file 5: Figure S4).
Chromatin association of dCTCF+, dCTCFΔC, and dCTCFΔN
As a prelude to analyzing the chromosome association of the mutant dCTCF proteins, we first examined the expression of transgenic wild-type and mutant dCTCF proteins. For this purpose we probed fly extracts with antibodies directed against the FLAG tag. As shown in the western blot in Additional file 8: Figure S7, the mutant proteins were expressed at nearly equivalent levels. When we probed western blots with antibodies directed against dCTCF, we found that the levels of proteins produced by the transgenes were about twofold less than that of the endogenous gene (not shown). This would suggest that the incomplete rescue of two of the GE24185 phenotypes (loss of A5 pigmentation and held out wings) by the hsp83:dCTCF + transgene is likely due, at least in part, to the insufficient expression of dCTCF.
Like dCTCF + , dCTCF ΔN and dCTCF ΔC occupancy at dCTCF sites in BX-C and the BGL3 insulators was reduced compared to wild type (Fig. 7). At most sites, the levels of dCTCF ΔN occupancy were very close to those for dCTCF + . In contrast, dCTCF ΔC occupancy levels were in most instances slightly lower (less than twofold) than either dCTCF + or dCTCF ΔN . Though the effects were small, they suggest that chromatin association of the dCTCF C-terminal deletion was partially compromised. Because this domain mediates interactions with CP190, this finding would support the idea that dCTCF binding to chromatin can be stabilized by interactions with CP190.
CP190 occupancy requires dCTCF but not necessarily the dCTCF-CTD
We also tested whether the reductions in CP190 occupancy evident in GE24185 mutants could be rescued by the hsp83:dCTCF transgenes encoding the wild-type and mutant proteins. Supporting the idea that dCTCF functions in CP190 recruitment, we found that dCTCF + and dCTCF ΔN promote CP190 occupancy at sites bound by dCTCF in vivo (Fig. 7). For BX-C insulators the effects on CP190 occupancy seemed to correlate with the levels of the transgene dCTCF associated with the insulator. For example, at Mcp where the dCTCF + and dCTCF ΔN transgene proteins were present at only about half the level of the endogenous dCTCF, CP190 occupancy was about 60 % of wild type (see Fig. 7). Similarly at Fab-8, the transgene dCTCF proteins and CP190 were present at levels about 30 % that of wild type. CP190 occupancy for two BGL3 insulators, 24C4 and 27B2, also depended upon dCTCF. In GE24185 mutants, CP190 was not detected at either of these insulators, while association was restored by the dCTCF + and, to a somewhat lesser extent, the dCTCF ΔN transgenes. Because the three other insulators (9A1, 21E2, and 57B1R) retained significant levels of both dCTCF and CP190 in GE24185 mutants, it was not clear whether dCTCF is essential for CP190 occupancy or if it is one of several factors that contribute to CP190-insulator association. Thus, though CP190 occupancy in dCTCF + and dCTCF ΔN flies at these three insulators was near wild type, the extent to which transgene dCTCF protein contributed to the rescue was not entirely clear.
Further insight into the role of dCTCF in CP190 occupancy came from ChIPs of dCTCF ΔC transgene embryos (Fig. 7). There seemed to be three classes with respect to the requirement for the dCTCF-CTD. In the first class were the BGL3 insulators 24C4 and 54B4R and also Fab-8. In this class, CP190 occupancy required the dCTCF-CTD and was substantially reduced in dCTCF ΔC transgene flies compared to wild type or the two other dCTCF rescue transgenes (Fig. 7). The second class was represented by Mcp and 27B2. Like 24C4, 54B4R and Fab-8, CP190 occupancy at these two insulators depended upon dCTCF and was reduced to near-background levels in GE24185 flies. However, unlike the insulators in the first class, the dCTCF ΔC transgene could partially rescue CP190 association. In the third class was the Abd-B promoter. Although CP190 occupancy at the Abd-B promoter required dCTCF (see Fig. 6), the requirement seemed to be independent of the dCTCF-CTD and was fully rescued by the dCTCF ΔC transgene.
dCTCF is required to properly initiate Abd-B expression in the embryo
The visible phenotypic defects in GE24185 adult flies arise from alterations in the patterns of gene expression induced by the gradual depletion of maternal dCTCF as the animals develop. It seemed possible that the effects on gene regulation might differ if dCTCF were completely absent at the onset of embryonic development instead of being present at near-normal levels and then slowly lost. To explore this possibility, we examined the expression of three genes, the homeotic gene Abd-B, the segment polarity gene, engrailed (en) , and the Notch pathway gene, insensitive (insv)  in the progeny of GE24185 mothers and fathers. Unlike the progeny of heterozygous parents, these embryos lack both maternal and zygotic dCTCF. Because the greatly reduced fecundity of GE24185 mothers made embryo collections problematic, we restricted our analysis to mid-embryogenesis.
The segment polarity gene en and the Notch pathway gene insv were expressed in a stripe-like pattern in each parasegment in the ectoderm of wild-type germ band extended embryos; however, while all of the cells in the en stripes appeared to express essentially the same levels of En protein (Fig. 9d,h) only a subset of the cells in the insv stripes expressed Insv (Figs. 8d and 9c). In the case of En, there were no obvious changes in the stripe pattern or in the level of protein in the cells expressing En in the dCTCF m-z- mutant. In contrast, there was a substantial increase in the number of cells that expressed Insv in dCTCF m-z- embryos. The level of Insv protein in these cells also appeared to be elevated. These changes were evident in both the stage 10 embryo in Fig. 8h and the stage 12 embryo in Fig. 9h.
We also examined the expression of Abd-B in GE24185 embryos rescued by the hsp83:dCTCF + , hsp83:dCTCF ΔN , and hsp83:dCTCF ΔC transgenes. The pattern of Abd-B expression in GE24185 hsp83:dCTCF + transgenic embryos resembled wild type. This was also true for GE24185 dCTCF ΔC transgenic embryos. In the case of hsp83:dCTCF ΔN , we occasionally observed stage 11–15 embryos that appeared to have slightly elevated levels of Abd-B.
CTCF is one of only a few insulator/chromosomal architectural DNA binding proteins that are known to be common to both insects and vertebrates [28, 30, 36, 85]. While it has been implicated in a wide range of nuclear functions ranging from gene expression to recombination and replication, a thread that seems common to most of its known activities is one of organizing the chromatin fiber. With the aim of better understanding its biological activities, we have begun a systematic analysis of the Drosophila melanogaster CTCF protein, dCTCF. In the studies reported here we have focused on two modules in the protein, one that mediates multimerization of dCTCF and the other that mediates interactions with the chromosomal protein CP190.
Using a combination of biochemical procedures we localized the dCTCF multimerization module to the N-terminus of the protein, spanning a region of about 100 amino acids between 70 and 163. At least part of this region (84–188) appears to be highly structured in solution as it is protease resistant. Although further studies will be required, our data would be most consistent with a model in which the multimerization module mediates the formation of tetrameric rather than dimeric dCTCF complexes. However, in either case, multimerization should enable dCTCF to interact simultaneously with several closely spaced recognition motifs because is found in insulators like Fab-3, Fab-6, and Fab-8. Presumably this could stabilize dCTCF association with chromatin by increasing the half-life of the protein–DNA complex. Alternatively, or in addition, the dCTCF multimers could bind simultaneously to dCTCF sites in different insulators, generating a chromatin loop linked together at its base by the insulator bound dCTCF multimeric complexes. A direct linkage of distant dCTCF binding sites by the dCTCF multimers would be consistent with the insulator bypass experiments using pairs of the appropriately oriented multimerized dCTCF binding sites . Like its insect counterpart, the vertebrate CTCF protein has also been implicated in the formation of chromatin loops . Because vertebrate CTCF is capable of self-association , it is possible that distant CTCF binding sites are linked together via some sort of CTCF multimeric complex. However, unlike the NTD of the Drosophila protein, biochemical studies suggest that the NTD of vertebrate CTCF is monomeric and disordered . Thus, multimer formation would have to involve other domains. In this regard it is of interest that Pant et al.  found pairwise interactions between the C-terminal zinc finger domains of the vertebrate CTCF protein.
We also analyzed how dCTCF and CP190 interact with each other. Yeast two-hybrid experiments and GST-pull-downs map the CP190 interaction module of dCTCF to the CTD. For CP190, the primary dCTCF interaction module was associated with the CP190 BTB domain. Previous studies have shown that the CP190 BTB domain forms a highly stable dimer [51, 61]. If there is a one-to-one relationship between the dCTCF-CTD–CP190 interaction module and the CP190 BTB domain, this CP190 BTB dimer could link two dCTCF multimers together, potentially building higher order protein–DNA complexes. However, our experiments do not favor a one-to-one interaction between dCTCF and CP190. Instead, our results would support a model in which a single dCTCF-CTD–CP190 interaction module associates preferentially with a dimerized CP190 BTB domain to give a 2xBTBCP190:dCTCF-CTD heterotrimer. This appears to be true, at least in these in vitro experiments, even when dCTCF is present in excess. Though confirmation will require a direct structural analysis of the dCTCF–CP190 complex, these findings would argue that the BTB dimer of CP190, by itself, could not function as the bridge linking two distant dCTCF insulators together. However, CP190 has additional domains (aspartic acid-rich D and microtubule targeting domains) that are involved in interaction with the DNA binding proteins like the insulator protein ZIPIC [60, 87]. Thus, CP190 might form complexes with or bridges between different insulator proteins like ZIPIC and dCTCF that might stabilize binding to chromatin. Because CP190 has a sequence non-specific zinc finger DNA BD , an alternative function for CP190 might be to stabilize dCTCF association with chromatin by binding (non-specifically) to sequences adjacent to dCTCF binding sites. However, because there are only very modest effects at most on dCTCF occupancy when the C-terminal dCTCF CP190 interaction domain is deleted, such a function would not seem to be critical.
To complement these biochemical studies, we examined the functioning of the dCTCF multimerization and CP190 interaction modules in vivo. Previous studies have shown that maternally derived dCTCF is sufficient to sustain development through to the adult stage . However, the viability of homozygous GE24185 animals, which lack zygotic dCTCF, was significantly reduced and the surviving adult flies exhibited several characteristic phenotypes. These included thin bristles, held out wings, abdominal segmentation defects, a substantially reduced egg production, and a maternal effect lethality. We found that an hsp83 transgene expressing the wild-type protein rescued all but two of the phenotypic effects of the GE24185 mutation. The two exceptions were the held out wing phenotype and the presence, in a small fraction of the hsp83:dCTCF WT adults, of a weak A5 (PS10) to A4 (PS9) transformation. It seems likely these two remaining phenotypes were due to the fact that the level of dCTCF expressed by the transgene is less than that produced by the endogenous gene. In the case of the A5 to A4 transformation, this suggestion fits with both the roughly tenfold reduction in dCTCF occupancy at the Fab-6 insulator and the known role of this insulator in Abd-B regulation. Fab-6 functions to protect the Abd-B cis-regulatory domain specifying PS10 (A5), iab-5, from the adjacent PS11 cis-regulatory domain, iab-6 [69, 77, 88, 89]. When only reduced levels of dCTCF are present, it is possible that Polycomb group (PcG) complexes may spread into iab-5 from iab-6 silencing the iab-5 cis-regulatory domain inappropriately in PS10.
Somewhat surprisingly, a dCTCF protein, dCTCFΔC, lacking sequences in the CTD critical for interactions with CP190 was just about as effective as the wild-type protein in complementing the GE24185 mutant. Like wild type, it rescued the zygotic and maternal effect lethality, the egg production defect, and the bristle phenotypes, but not the held out wing or the A5 to A4 transformation. ChIP experiments were consistent with the idea that CP190 interactions may help stabilize dCTCF association with chromatin because dCTCF occupancy levels were reduced in hsp83:dCTCF ΔC pupae (compared to hsp83:dCTCF WT ) at most of the insulators we examined. While the effects of the mutation on dCTCF occupancy were modest, much greater reductions in CP190 levels were evident at least at some sites (e.g., the BGL3 insulators, 24C and 57B4R; Fig. 6). At these sites it would appear that CP190 occupancy required the dCTCF C-terminal CP190 interaction module. However, this was not always the case. For example, at Mcp, the presence of the mutant dCTCFΔC protein was sufficient to ensure CP190 occupancy (Fig. 6). Because CP190 occupancy at Mcp required dCTCF (see Fig. 5), it would appear that dCTCF can promote CP190 association by a mechanism that is independent of its C-terminal CP190 interaction module. Recently, Pita was found to bind Mcp and recruit CP190 . The binding of the two proteins to Mcp are interdependent. One obvious possibility is that dCTCFΔC binding to Mcp stabilizes the association of Pita, which in turn can recruit CP190.
While the dCTCFΔC protein was just about as effective as wild-type dCTCF in rescuing the GE24185, this was not true for dCTCFΔN. This mutant protein only partially ameliorated the zygotic and maternal effect lethality, and surviving adult flies exhibited the same visible phenotypes as the GE24185 mutant. Because occupancy levels of the dCTCFΔN protein were nearly equivalent to that of dCTCFWT, it would appear that some other activity of the dCTCF protein must be partially compromised by the deletion of the multimerization domain. One possibility would be a function in linking distant dCTCF insulators together; however, this region of the protein could have other activities besides mediating dCTCF dimerization. Importantly, because CP190 occupancy at BX-C insulators and the Abd-B promoter was similar to that in hsp83:dCTCF WT , Abd-B-dependent abdominal phenotypes in adult hsp83:dCTCF ΔN flies (and, by inference, GE24185 flies) may arise by a mechanism that is independent of CP190.
Because there is a substantial maternal contribution of dCTCF, the abdominal segmentation defects in surviving GE24185 flies were likely a consequence of a gradual reduction in dCTCF occupancy at the Abd-B insulators and/or promoter as the animals developed. Significantly, eliminating dCTCF at the onset of embryonic development had a quite different effect on Abd-B gene activity. Instead of being reduced, Abd-B expression was substantially upregulated in the posterior parasegments of dCTCF m-z- embryos. These paradoxical effects on Abd-B regulation would not be consistent with a primary function for dCTCF in the intrinsic activity of the Abd-B promoter—the adult phenotype would require dCTCF to function as an activator, while the embryonic phenotype would require dCTCF to function as a repressor. Rather, one would imagine that whatever role dCTCF plays at the Abd-B promoter (positive or negative), this function is likely to be the same throughout development. For this reason, the opposing stage-specific effects on Abd-B activity are more readily explained by the quite different modes of regulation of the Abd-B (iab) cis-regulatory domains in embryos and in larvae/pupae [88, 89].
In larvae and pupae, Abd-B regulation is in the maintenance phase and depends upon the PREs in each iab cis-regulatory domain [73, 88–91]. The PREs function to recruit PcG proteins, keeping inactive cis-regulatory domains off in the parasegments where they should be silenced. For this reason, a gradual reduction in insulator activity in the larval and pupal stages could result in the spreading of PcG silencing from PREs in inactive cis-regulatory domains to their flanking active neighbors and the consequent downregulation of Abd-B gene activity in posterior parasegments. (Of course, if dCTCF were also required for Abd-B promoter activity, this would tend to enhance any loss-of-function phenotypes associated with the spread of silencing in the cis-regulatory domains in dCTCF z- mutants. Conversely, these loss-of-function phenotypes would be suppressed if dCTCF represses instead of enhances Abd-B promoter activity). By contrast, in dCTCF m-z- embryos, dCTCF would be absent when the gap and pair-rule genes initially establish the parasegment-specific patterns of Abd-B gene activity during the blastoderm–early gastrula stage [92–95]. At this point in development one of the key functions of insulators is to prevent cross talk between the adjacent parasegment-specific initiation elements that can inappropriately activate or silence the cis-regulatory domains flanking the insulator [67, 69, 77, 96]. For mutations that disrupt specific insulators, this mix of ectopic activation and silencing can be visualized in the embryo by changes in the level of Abd-B expression [67, 77, 96]. For example, in Fab-6 mutants, the inappropriate activation of the iab-6 cis-regulatory domain in PS10 would noticeably upregulate Abd-B expression in this parasegment compared to that in wild type where iab-5 normally directs Abd-B expression . However, the level of Abd-B expression in PS10 in the Fab-6 mutant is not equivalent to that in the adjacent parasegment PS11 because in some of the PS10 cells the iab-5 cis-regulatory domain is inappropriately silenced. In fact, this is the phenotype that we observed in dCTCF m-z- mutant embryos. Abd-B expression is each posterior parasegment was upregulated compared to the corresponding parasegment in wild type, but the extent of upregulation was not equivalent to that in the adjacent more posterior parasegments.
While the opposing phenotypes associated with the loss dCTCF activity at different stages of development argue in favor of the idea that dCTCF is critical to the functioning of the insulators associated with the Abd-B cis-regulatory domains, there was one rather puzzling observation—namely, the apparent effects of the dCTCF GE24185 mutation on the activity of the Fab-7 insulator. In adult GE24185 flies, the partial transformation of A6 (PS11) into A5 (PS10) would point either to a loss of Abd-B promoter activity or the spreading of silencing from iab-7 PRE into iab-6, shutting off this cis-regulatory domain in PS10 (or a combination of both). In the embryo, the upregulation of Abd-B expression in PS11 would require either an increase in Abd-B promoter activity or the ectopic activation of iab-7 by the iab-6 initiator in PS11 cells. While these stage-specific phenotypic effects are more readily explained by a disruption in Fab-7 insulator activity rather than diametrically opposite effects on the Abd-B promoter as development proceeds, Fab-7 differs from the other Abd-B insulators in that it does not have dCTCF binding sites . In this case, the apparent loss of Fab-7 insulator activity would have to be an indirect consequence of disruptions in the functioning of the neighboring dCTCF-dependent insulators, Fab-6 and Fab-8. Although transgene assays have argued that the activity of an insulator depends upon its neighbors (for review see ), this would be one of the first examples in an endogenous setting. Further studies will clearly be required to explain the apparent effects on Fab-7 activity.
Additionally, we found that the expression of the Notch pathway gene, insv, was also changed in dCTCF m-z- embryos, while en expression seemed to be unaffected. Unlike Abd-B, where there are good reasons to believe that the effects on gene regulation are a direct consequence of the loss of dCTCF activity, we do not know whether the effects on insv are direct or indirect. However, it is worth noting that the insv gene and its partner, elba2, are flanked by sequences that are bound by dCTCF, while the potential insulators for the en locus appear to be occupied by Su(Hw) rather than dCTCF [56, 57].
CTCF is one of the few DNA binding insulator proteins that is conserved in bilaterians. To learn more about its role in chromosome architecture we have identified and characterized the two protein–protein interaction modules in Drosophila dCTCF. The first is responsible for the multimerization of the dCTCF protein while the second is responsible for interactions between dCTCF and CP190. We have also tested the functioning of proteins lacking these interaction modules in vivo. We found that a dCTCF protein lacking sequences critical for CP190 interactions was almost as effective as wild type in rescuing the phenotypic effects of a dCTCF null allele. In contrast, a dCTCF protein lacking the multimerization domain had only partial functionality and did not fully rescue phenotypic effects of the null allele.
For protein purification purposes, protein fragments were either PCR-amplified using corresponding primers (see Additional file 9: Table S2), or digested from dCTCF cDNA (1–288, BamHI-EcoRI; 1–205, BamHI-XhoI; 1–163, BamHI-PvuII; 1–125, BamHI-RsaI; 70–163, MboI-PvuII; 125–180, RsaI-RsaI) and subcloned into pGEX-4T1 (GE Healthcare, Little Chalfont, Buckinghamshire, United Kingdom) or pET32a(+) vector (Merck Biosciences, Darmstadt, Germany) in-frame with corresponding tag. In the case of 6xHis-fusions without thioredoxin, its coding sequence was excised from pET32a(+) vector with NdeI. A modified pET32a(+) vector was used to express proteins with a TEV-cleavable thioredoxin-6xHis-tag.
For protein expression in S2 cells and for generation of transgenic flies, protein coding sequences were cloned in-frame with 3xFLAG, excised, and subcloned into the Casper vector with Hsp83 promoter .
Generation and analysis of transgenic lines
The construct and P25.7wc plasmid were injected into yacw 1118 pre-blastoderm embryos . The resultant flies were crossed with yacw 1118 flies, and the transgenic progeny were identified by their eye color.
Protein expression and purification, size-exclusion chromatography, and chemical cross-linking
Protein expression and purification were performed using standard procedures. Briefly, BL21 cells were disrupted by sonication in buffer A (40 mM HEPES-KOH pH 7.7, 400 mM NaCl, 5 mM β-mercaptoethanol, 20 mM imidazole) containing 1 mM PMSF and Calbiochem Complete Protease Inhibitor Cocktail VII (1 μL/1 ml). After centrifugation, lysate was applied to an Ni-NTA column, and, after washing, was eluted with 300 mM imidazole and dialyzed against an appropriate buffer. For cleavage of the thioredoxin-6xHis-tagged protein, TEV protease was added at a molar ratio of 1:50 directly to the eluted protein. The mixture was incubated for 2 h at room temperature, dialyzed against buffer A, and applied to the Ni-NTA column. Flow-through was collected; dialyzed against 20 mM Tris–HCl pH 7.4, and 1 mM DTT; and further purified using a SOURCE15Q 4.6/100 column (GE Healthcare). Size-exclusion chromatography was performed as described  using Sephacryl S200 16/60 or Superdex 200 10/300GL columns (GE Healthcare) in 20 mM Tris–HCl pH 7.4, 150 mM NaCl, and 1 mM DTT. The protein concentration was adjusted to 5 μM and chemical cross-linking was carried out for 10 min at room temperature in buffer B containing 20 mM HEPES-KOH pH 7.7, 150 mM NaCl, 20 mM imidazole, and 1 mM β-mercaptoethanol. Cross-linking was quenched with 50 mM glycine and the cross-linked samples were resolved using SDS-PAGE followed by silver-staining. For analysis of complex formation between CP190-BTB and CTCF-CTD, the proteins were purified, dialyzed against buffer B, and mixed in corresponding molar ratio, with a constant CP190-BTB concentration of 20 μM. After incubation at room temperature for 2 h the protein mixture was cross-linked with glutaraldehyde as described above. Samples were resolved using SDS-PAGE followed by Coomassie staining.
GST-pull-downs were performed with Immobilized Glutathione Agarose (Pierce) in buffer C (20 mM HEPES-KOH pH 7.7, 150 mM NaCl, 10 mM MgCl2, 0.1 mM ZnCl2, 0.1 % NP40, 10 % (w/w) glycerol). BL21 cells were grown in lysogeny broth media to an A600 of 1.0 at 37 °C and then induced with 1 mM IPTG at 18 °C overnight. ZnCl2 was added to a final concentration of 100 μM before induction. Cells were disrupted by sonication, centrifuged, and applied to resin for 10 min at room temperature. After binding, the resin was washed two times with buffer C. The resin with immobilized protein was then mixed with a solution of interacting protein or Schneider 2 cells nuclear lysate equilibrated in buffer C. Incubation was continued for 2 h at room temperature (for recombinant proteins) or at +4 °C (Schneider 2 cells nuclear lysate). The resin was then washed four times with buffer C containing 500 mM NaCl and elution performed with 50 mM reduced glutathione and 100 mM Tris pH 8.0, for 15 min.
Thioredoxin-fused CTCF[1–205] protein was purified and dialyzed against 20 mM HEPES-KOH pH 7.7, 150 mM NaCl, 10 mM MgCl2, and 2.5 mM CaCl2. The protein concentration was adjusted to 10 μM and indicated amounts of proteinase K (Fermentas) or trypsin (Sigma) diluted in the same buffer were added. After 10 min incubation at room temperature, PMSF was added to final concentration of 5 mM and incubation continued for a further 10 min. Samples were resolved using SDS-PAGE followed by Coomassie staining. The protein bands were excised and subjected to complete trypsin digestion and MALDI-TOF mass spectrometry. Protein identification and peptide mapping were performed using the MASCOT server (Matrix Science).
Drosophila cells nuclear lysate preparation
Schneider 2 cells were grown in SFX media, collected by centrifugation at 700 g for 5 min, washed once with 1xPBS, resuspended in buffer IP-0 (10 mМ Tris–HCl pH 8.0, 10 mМ NaCl, 10 mМ MgCl2, 1 mМ EDTA, 1 mМ EGTA, 1 mМ DTT, 250 mМ sucrose, 1 mМ PMSF, 0.2 % NP-40, Calbiochem Protease Inhibitor Cocktail V), incubated for 10 minutes at +4 °C, and disrupted by 20 strokes in Dounce homogenizer on ice. Nuclei were collected at 3000 g for 10 min and resuspended in IP-10+ (10 mМ Tris–HCl pH 8.0, 10 mМ NaCl, 10 mМ MgCl2, 1 mМ EDTA, 1 mМ EGTA, 1 mМ DTT, 10 % glycerol, 0.1 % NP-40; in the case of subsequent immunoprecipitation assays, DTT was not used). An equal volume of IP-850+ (10 mМ Tris–HCl pH 8.0, 850 mМ NaCl, 10 mМ MgCl2, 1 mМ EDTA, 1 mМ EGTA, 1 mМ DTT, 10 % glycerol, 0.1 % NP-40) was added and nuclei were lysed for 10 min on ice, after that two volumes of IP-10+ and 1 U/ml DNAse I were added and incubation was continued for 10 min in a rotator at room temperature. After centrifugation at 16,000 g for 20 min, lysate was used in immunoprecipitation or GST-pull-down assays.
Antibodies were immobilized for 2 h on pre-equilibrated Protein A or Protein G beads in buffer D (20 mM Tris pH 7.4, 150 mM NaCl, 10 mM MgCl2, 0.1 mM ZnCl2, 0.1 % NP40, 10 % (w/w) glycerol). The beads were then washed for 1 h with the same buffer containing 10 mg/ml BSA, and incubated overnight at +4 °C with nuclear lysate. After incubation with the lysate, the beads were washed four times with buffer D containing 500 mM NaCl and boiled in SDS-PAGE sample buffer.
Fly extract preparation
Twenty adult flies were homogenized with a pestle in 200 μl of 1xPBS containing 1 % β-mercaptoethanol, 10 mM PMSF, and 1:100 Calbiochem Complete Protease Inhibitor Cocktail VII. The suspension was sonicated three times for 5 s at 5 W. Then, 200 μl of 4xSDS-PAGE sample buffer was added and the mixture incubated for 10 min at 100 °C and centrifuged at 16,000 g for 10 min.
Antibodies were raised against dCTCF[1–163], dCTCF[612–818], and CP190[308–1096] fragments in rabbits and rats. GAGA-factor antibodies were raised against full-length protein in rats. Antibodies were purified from serum by ammonium sulfate fractionation followed by affinity purification using CNBr-activated Sepharose (GE Healthcare) with standard protocols. Other antibodies were anti-FLAG (M2, Sigma), anti-6xHis (GE Healthcare), anti-Abd-B (Iowa Developmental Studies Hybridoma Bank), and anti-Insv (gift of Tsutomu Aoki).
Cell culture, transfection, and dual luciferase assay
Drosophila S2 cells were grown in SFX medium (HyClone) at 25 °C. Transfection of plasmids was performed with the Cellfectin II reagent (Invitrogen) according to the manufacturer’s instructions. Typically, cells were transfected in six-well plates and grown for 24–48 h before harvesting. All transfection procedures were performed with three independent replicates.
The dual luciferase assay was performed with the Firefly & Renilla Luciferase Assay Kit (Biotium).
Chromatin was prepared from S2 cells and mid-late pupae.
Formaldehyde from 10 % stock was added to a 1 % final concentration to 107 cells in 10 ml of SFX medium and samples were incubated in a rotator at room temperature for 15 min. The cross-linking was stopped by 0.125 M glycine. The samples were placed on ice and washed three times with PBS with 0.5 mM PMSF and centrifuged at 1000 rpm for 5 min at 4 °C. The pellet was resuspended in 10 ml of buffer I (25 mM HEPES pH 7.8, 1.5 mM MgCl2, 10 mM KCl, 0.1 % NP40, 1 mM DTT, 0.5 mM PMSF, Calbiochem Complete (EDTA)-free Protease Inhibitor Cocktail V) and placed on ice for 10 min. The suspension was homogenized in a Dounce homogenizer with pestle “B” 20 times and centrifuged at 2000 rpm for 5 min at 4 °C. The pellet was resuspended in 3 ml of buffer II (50 mM HEPES pH 7.8, 140 mM NaCl, 1 mM EDTA, 1 % Triton X-100, 0.1 % sodium deoxycholate, 0.1 % SDS, 0.5 mM PMSF, Calbiochem Complete (EDTA)-free Protease Inhibitor Cocktail V) and sonicated in a Bioruptor sonifier (40 alternating 30-s ON and 60-s OFF intervals). Finally, 50-μL aliquots were used to test the extent of sonication and to measure DNA concentration.
A 500-mg pupa sample was ground in a mortar in liquid nitrogen and resuspended in 10 mL of buffer A (15 mM HEPES-KOH pH 7.6, 60 mM KCl, 15 mM NaCl, 13 mM EDTA, 0.1 mM EGTA, 0.15 mM spermine, 0.5 mM spermidine, 0.5 % NP-40, 0.5 mM DTT) supplemented with 0.5 mM PMSF and Calbiochem Protease Inhibitor Cocktail V. The suspension was then homogenized in a Dounce homogenizer with pestle “B” and filtered through Nylon Cell Strainer (BD Biosciences, USA). The homogenate was transferred to 3 mL of buffer A with 10 % sucrose (AS), and the nuclei were pelleted by centrifugation at 4000 g for 5 min at 4 °C. The pellet was resuspended in 5 mL of buffer A, homogenized again in a Dounce homogenizer, and transferred to 1.5 mL of buffer AS to collect the nuclei by centrifugation. The nuclear pellet was resuspended in wash buffer (15 mM HEPES-KOH pH 7.6, 60 mM KCl, 15 mM NaCl, 1 mM EDTA, 0.1 mM EGTA, 0.1 % NP-40, protease inhibitors) and cross-linked with 1 % formaldehyde for 15 min at room temperature. Cross-linking was stopped by adding glycine to a final concentration of 125 mM. The nuclei were washed with three 10-mL portions of wash buffer and resuspended in 1.5 mL of nuclear lysis buffer (15 mM HEPES pH 7.6, 140 mM NaCl, 1 mM EDTA, 0.1 mM EGTA, 1 % Triton X-100, 0.5 mM DTT, 0.1 % sodium deoxycholate, 0.1 % SDS, protease inhibitors). The suspension was sonicated in a Bioruptor sonifier (35 alternating 30-s ON and 60-s OFF intervals), and 50-μL aliquots were used to test the extent of sonication and to measure DNA concentration.
Debris was removed by centrifugation at 14,000 g for 10 min at 4 °C, and chromatin was pre-cleared with Protein A agarose (Pierce) blocked with BSA and salmon sperm DNA, with 50-μL aliquots of such pre-cleared chromatin being stored as input material. Samples containing 10–20 μg of DNA equivalent in 1 mL of nuclear lysis buffer were incubated overnight at 4 °C with rabbit antibodies against dCTCF (1:500) and CP190 (1:1000), mouse antibodies against FLAGx3 (1:200), or with non-specific IgG purified from rabbit or mouse pre-immune sera (control). Chromatin–antibody complexes were collected using blocked Protein A or G agarose at 4 °C over 5 h. After several rounds of washing with lysis buffer (as such and with 500 mM NaCl), LiCl buffer (20 mM Tris–HCl pH 8, 250 mM LiCl, 1 mM EDTA, 0.5 % NP-40, 0.5 % sodium deoxycholate, protease inhibitors), and TE buffer (10 mM Tris–HCl pH 8, 1 mM EDTA), the DNA was eluted with elution buffer (50 mM Tris–HCl, pH 8.0, 1 mM EDTA, 1 % SDS), the cross-links were reversed, and the precipitated DNA was extracted by the phenol–chloroform method. The enrichment of specific DNA fragments was analyzed by real-time PCR, using a StepOne Plus Thermal Cycler (Applied Biosystems). The primers used for PCR in ChIP experiments for genome fragments are shown in Additional file 9: Table S2.
The authors would like to thank Tsutomu Aoki for the gift of Insv polyclonal antibodies. This study was supported by the Ministry of Education and Science of the Russian Federation (project no. 14.B25.31.0022) and a grant from NIH GM043432 to PS, and Russian Foundation for Basic Research (projects no. 13-04-00340 and 13-04-40136-n) and RF Presidential Stipend no. SP- 4081.2015.4 to OM.
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- Chetverina D, Aoki T, Erokhin M, Georgiev P, Schedl P. Making connections: insulators organize eukaryotic chromosomes into independent cis-regulatory networks. Bioessays. 2014;36:163–72.PubMed CentralPubMedView ArticleGoogle Scholar
- Tanay A, Cavalli G. Chromosomal domains: epigenetic contexts and functional implications of genomic compartmentalization. Curr Opin Genet Dev. 2013;23:197–203.PubMedView ArticleGoogle Scholar
- Krijger PH, de Laat W. Identical cells with different 3D genomes; cause and consequences? Curr Opin Genet Dev. 2013;23:191–6.PubMedView ArticleGoogle Scholar
- Wendt KS, Grosveld FG. Transcription in the context of the 3D nucleus. Curr Opin Genet Dev. 2014;25:62–7.PubMedView ArticleGoogle Scholar
- Nora EP, Dekker J, Heard E. Segmental folding of chromosomes: a basis for structural and regulatory chromosomal neighborhoods? Bioessays. 2013;35:818–28.PubMed CentralPubMedView ArticleGoogle Scholar
- Gibcus JH, Dekker J. The hierarchy of the 3D genome. Mol Cell. 2013;49:773–82.PubMed CentralPubMedView ArticleGoogle Scholar
- Kuhn EJ, Geyer PK. Genomic insulators: connecting properties to mechanism. Curr Opin Cell Biol. 2003;15:259–65.PubMedView ArticleGoogle Scholar
- Brasset E, Vaury C. Insulators are fundamental components of the eukaryotic genomes. Heredity. 2005;94:571–6.PubMedView ArticleGoogle Scholar
- Raab JR, Kamakaka RT. Insulators and promoters: closer than we think. Nat Rev Genet. 2010;11:439–46.PubMed CentralPubMedView ArticleGoogle Scholar
- Matzat LH, Lei EP. Surviving an identity crisis: a revised view of chromatin insulators in the genomics era. Biochim Biophys Acta. 1839;2014:203–14.Google Scholar
- Schoborg T, Labrador M. Expanding the roles of chromatin insulators in nuclear architecture, chromatin organization and genome function. Cell Mol Life Sci. 2014;71:4089–113.PubMedView ArticleGoogle Scholar
- Kyrchanova O, Georgiev P. Chromatin insulators and long-distance interactions in Drosophila. FEBS Lett. 2014;588:8–14.PubMedView ArticleGoogle Scholar
- Maksimenko O, Georgiev P. Mechanisms and proteins involved in long-distance interactions. Front Genet. 2014;5:28.PubMed CentralPubMedView ArticleGoogle Scholar
- Cleard F, Moshkin Y, Karch F, Maeda RK. Probing long-distance regulatory interactions in the Drosophila melanogaster bithorax complex using Dam identification. Nat Genet. 2006;38:931–5.PubMedView ArticleGoogle Scholar
- Kyrchanova O, Chetverina D, Maksimenko O, Kullyev A, Georgiev P. Orientation-dependent interaction between Drosophila insulators is a property of this class of regulatory elements. Nucleic Acids Res. 2008;36:7019–28.PubMed CentralPubMedView ArticleGoogle Scholar
- Kyrchanova O, Ivlieva T, Toshchakov S, Parshikov A, Maksimenko O, Georgiev P. Selective interactions of boundaries with upstream region of Abd-B promoter in Drosophila bithorax complex and role of dCTCF in this process. Nucleic Acids Res. 2011;39:3042–52.PubMed CentralPubMedView ArticleGoogle Scholar
- Fujioka M, Sun G, Jaynes JB. The Drosophila eve insulator Homie promotes eve expression and protects the adjacent gene from repression by polycomb spreading. PLoS Genet. 2013;9:e1003883.PubMed CentralPubMedView ArticleGoogle Scholar
- Junier I, Dale RK, Hou C, Kepes F, Dean A. CTCF-mediated transcriptional regulation through cell type-specific chromosome organization in the beta-globin locus. Nucleic Acids Res. 2012;40:7718–27.PubMed CentralPubMedView ArticleGoogle Scholar
- Kim A, Dean A. Chromatin loop formation in the beta-globin locus and its role in globin gene transcription. Mol Cells. 2012;34:1–5.PubMed CentralPubMedView ArticleGoogle Scholar
- Erokhin M, Davydova A, Kyrchanova O, Parshikov A, Georgiev P, Chetverina D. Insulators form gene loops by interacting with promoters in Drosophila. Development. 2011;138:4097–106.PubMedView ArticleGoogle Scholar
- Kyrchanova O, Maksimenko O, Stakhov V, Ivlieva T, Parshikov A, Studitsky VM, et al. Effective blocking of the white enhancer requires cooperation between two main mechanisms suggested for the insulator function. PLoS Genet. 2013;9:e1003606.PubMed CentralPubMedView ArticleGoogle Scholar
- Bell AC, West AG, Felsenfeld G. The protein CTCF is required for the enhancer blocking activity of vertebrate insulators. Cell. 1999;98:387–96.PubMedView ArticleGoogle Scholar
- Gray CE, Coates CJ. Cloning and characterization of cDNAs encoding putative CTCFs in the mosquitoes, Aedes aegypti and Anopheles gambiae. BMC Mol Biol. 2005;6:16.PubMed CentralPubMedView ArticleGoogle Scholar
- Heger P, Marin B, Schierenberg E. Loss of the insulator protein CTCF during nematode evolution. BMC Mol Biol. 2009;10:84.PubMed CentralPubMedView ArticleGoogle Scholar
- Heger P, Marin B, Bartkuhn M, Schierenberg E, Wiehe T. The chromatin insulator CTCF and the emergence of metazoan diversity. Proc Natl Acad Sci U S A. 2012;109:17507–12.PubMed CentralPubMedView ArticleGoogle Scholar
- Filippova GN, Fagerlie S, Klenova EM, Myers C, Dehner Y, Goodwin G, et al. An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes. Mol Cell Biol. 1996;16:2802–13.PubMed CentralPubMedGoogle Scholar
- Klenova EM, Nicolas RH, Paterson HF, Carne AF, Heath CM, Goodwin GH, et al. CTCF, a conserved nuclear factor required for optimal transcriptional activity of the chicken c-myc gene, is an 11-Zn-finger protein differentially expressed in multiple forms. Mol Cell Biol. 1993;13:7612–24.PubMed CentralPubMedGoogle Scholar
- Merkenschlager M, Odom DT. CTCF and cohesin: linking gene regulatory elements with their targets. Cell. 2013;152:1285–97.PubMedView ArticleGoogle Scholar
- Ohlsson R, Lobanenkov V, Klenova E. Does CTCF mediate between nuclear organization and gene expression? Bioessays. 2010;32:37–50.PubMedView ArticleGoogle Scholar
- Ghirlando R, Giles K, Gowher H, Xiao T, Xu Z, Yao H, et al. Chromatin domains, insulators, and the regulation of gene expression. Biochim Biophys Acta. 1819;2012:644–51.Google Scholar
- Zuin J, Dixon JR, van der Reijden MI, Ye Z, Kolovos P, Brouwer RW, et al. Cohesin and CTCF differentially affect chromatin architecture and gene expression in human cells. Proc Natl Acad Sci U S A. 2014;111:996–1001.PubMed CentralPubMedView ArticleGoogle Scholar
- Handoko L, Xu H, Li G, Ngan CY, Chew E, Schnapp M, et al. CTCF-mediated functional chromatin interactome in pluripotent cells. Nat Genet. 2011;43:630–8.PubMed CentralPubMedView ArticleGoogle Scholar
- Dixon JR, Selvaraj S, Yue F, Kim A, Li Y, Shen Y, et al. Topological domains in mammalian genomes identified by analysis of chromatin interactions. Nature. 2012;485:376–80.PubMed CentralPubMedView ArticleGoogle Scholar
- Sanyal A, Lajoie BR, Jain G, Dekker J. The long-range interaction landscape of gene promoters. Nature. 2012;489:109–13.PubMed CentralPubMedView ArticleGoogle Scholar
- Kyrchanova O, Toshchakov S, Podstreshnaya Y, Parshikov A, Georgiev P. Functional interaction between the Fab-7 and Fab-8 boundaries and the upstream promoter region in the Drosophila Abd-B gene. Mol Cell Biol. 2008;28:4188–95.PubMed CentralPubMedView ArticleGoogle Scholar
- Herold M, Bartkuhn M, Renkawitz R. CTCF: insights into insulator function during development. Development. 2012;139:1045–57.PubMedView ArticleGoogle Scholar
- Moon H, Filippova G, Loukinov D, Pugacheva E, Chen Q, Smith ST, et al. CTCF is conserved from Drosophila to humans and confers enhancer blocking of the Fab-8 insulator. EMBO Rep. 2005;6:165–70.PubMed CentralPubMedView ArticleGoogle Scholar
- Wendt KS, Yoshida K, Itoh T, Bando M, Koch B, Schirghuber E, et al. Cohesin mediates transcriptional insulation by CCCTC-binding factor. Nature. 2008;451:796–801.PubMedView ArticleGoogle Scholar
- Parelho V, Hadjur S, Spivakov M, Leleu M, Sauer S, Gregson HC, et al. Cohesins functionally associate with CTCF on mammalian chromosome arms. Cell. 2008;132:422–33.PubMedView ArticleGoogle Scholar
- Rubio ED, Reiss DJ, Welcsh PL, Disteche CM, Filippova GN, Baliga NS, et al. CTCF physically links cohesin to chromatin. Proc Natl Acad Sci U S A. 2008;105:8309–14.PubMed CentralPubMedView ArticleGoogle Scholar
- Stedman W, Kang H, Lin S, Kissil JL, Bartolomei MS, Lieberman PM. Cohesins localize with CTCF at the KSHV latency control region and at cellular c-myc and H19/Igf2 insulators. Embo J. 2008;27:654–66.PubMed CentralPubMedView ArticleGoogle Scholar
- Heidari N, Phanstiel DH, He C, Grubert F, Jahanbani F, Kasowski M, et al. Genome-wide map of regulatory interactions in the human genome. Genome Res. 2014;24:1905–17.PubMed CentralPubMedView ArticleGoogle Scholar
- Jin F, Li Y, Dixon JR, Selvaraj S, Ye Z, Lee AY, et al. A high-resolution map of the three-dimensional chromatin interactome in human cells. Nature. 2013;503:290–4.PubMedGoogle Scholar
- Dorsett D. Cohesin: genomic insights into controlling gene transcription and development. Curr Opin Genet Dev. 2011;21:199–206.PubMed CentralPubMedView ArticleGoogle Scholar
- Xiao T, Wallace J, Felsenfeld G. Specific sites in the C terminus of CTCF interact with the SA2 subunit of the cohesin complex and are required for cohesin-dependent insulation activity. Mol Cell Biol. 2011;31:2174–83.PubMed CentralPubMedView ArticleGoogle Scholar
- Misulovin Z, Schwartz YB, Li XY, Kahn TG, Gause M, MacArthur S, et al. Association of cohesin and Nipped-B with transcriptionally active regions of the Drosophila melanogaster genome. Chromosoma. 2008;117:89–102.PubMed CentralPubMedView ArticleGoogle Scholar
- Ahanger SH, Srinivasan A, Vasanthi D, Shouche YS, Mishra RK. Conserved boundary elements from the Hox complex of mosquito, Anopheles gambiae. Nucleic Acids Res. 2013;41:804–16.PubMed CentralPubMedView ArticleGoogle Scholar
- Whitfield WG, Chaplin MA, Oegema K, Parry H, Glover DM. The 190 kDa centrosome-associated protein of Drosophila melanogaster contains four zinc finger motifs and binds to specific sites on polytene chromosomes. J Cell Sci. 1995;108:3377–87.PubMedGoogle Scholar
- Kellogg DR, Field CM, Alberts BM. Identification of microtubule-associated proteins in the centrosome, spindle, and kinetochore of the early Drosophila embryo. J Cell Biol. 1989;109:2977–91.PubMedView ArticleGoogle Scholar
- Butcher RD, Chodagam S, Basto R, Wakefield JG, Henderson DS, Raff JW, et al. The Drosophila centrosome-associated protein CP190 is essential for viability but not for cell division. J Cell Sci. 2004;117:1191–9.PubMedView ArticleGoogle Scholar
- Vogelmann J, Le Gall A, Dejardin S, Allemand F, Gamot A, Labesse G, et al. Chromatin insulator factors involved in long-range DNA interactions and their role in the folding of the Drosophila genome. PLoS Genet. 2014;10:e1004544.PubMed CentralPubMedView ArticleGoogle Scholar
- Pai CY, Lei EP, Ghosh D, Corces VG. The centrosomal protein CP190 is a component of the gypsy chromatin insulator. Mol Cell. 2004;16:737–48.PubMedView ArticleGoogle Scholar
- Moshkovich N, Nisha P, Boyle PJ, Thompson BA, Dale RK, Lei EP. RNAi-independent role for Argonaute2 in CTCF/CP190 chromatin insulator function. Genes Dev. 2011;25:1686–701.PubMed CentralPubMedView ArticleGoogle Scholar
- Gerasimova TI, Lei EP, Bushey AM, Corces VG. Coordinated control of dCTCF and gypsy chromatin insulators in Drosophila. Mol Cell. 2007;28:761–72.PubMed CentralPubMedView ArticleGoogle Scholar
- Mohan M, Bartkuhn M, Herold M, Philippen A, Heinl N, Bardenhagen I, et al. The Drosophila insulator proteins CTCF and CP190 link enhancer blocking to body patterning. EMBO J. 2007;26:4203–14.PubMed CentralPubMedView ArticleGoogle Scholar
- Schwartz YB, Linder-Basso D, Kharchenko PV, Tolstorukov MY, Kim M, Li HB, et al. Nature and function of insulator protein binding sites in the Drosophila genome. Genome Res. 2012;22:2188–98.PubMed CentralPubMedView ArticleGoogle Scholar
- Negre N, Brown CD, Shah PK, Kheradpour P, Morrison CA, Henikoff JG, et al. A comprehensive map of insulator elements for the Drosophila genome. PLoS Genet. 2010;6:e1000814.PubMed CentralPubMedView ArticleGoogle Scholar
- Yusufzai TM, Tagami H, Nakatani Y, Felsenfeld G. CTCF tethers an insulator to subnuclear sites, suggesting shared insulator mechanisms across species. Mol Cell. 2004;13:291–8.PubMedView ArticleGoogle Scholar
- Pant V, Kurukuti S, Pugacheva E, Shamsuddin S, Mariano P, Renkawitz R, et al. Mutation of a single CTCF target site within the H19 imprinting control region leads to loss of Igf2 imprinting and complex patterns of de novo methylation upon maternal inheritance. Mol Cell Biol. 2004;24:3497–504.PubMed CentralPubMedView ArticleGoogle Scholar
- Oliver D, Sheehan B, South H, Akbari O, Pai CY. The chromosomal association/dissociation of the chromatin insulator protein Cp190 of Drosophila melanogaster is mediated by the BTB/POZ domain and two acidic regions. BMC Cell Biol. 2010;11:101.PubMed CentralPubMedView ArticleGoogle Scholar
- Bonchuk A, Denisov S, Georgiev P, Maksimenko O. Drosophila BTB/POZ domains of "ttk group" can form multimers and selectively interact with each other. J Mol Biol. 2011;412:423–36.PubMedView ArticleGoogle Scholar
- Ahmad KF, Melnick A, Lax S, Bouchard D, Liu J, Kiang CL, et al. Mechanism of SMRT corepressor recruitment by the BCL6 BTB domain. Mol Cell. 2003;12:1551–64.PubMedView ArticleGoogle Scholar
- MacDonald WA, Menon D, Bartlett NJ, Sperry GE, Rasheva V, Meller V, et al. The Drosophila homolog of the mammalian imprint regulator, CTCF, maintains the maternal genomic imprint in Drosophila melanogaster. BMC Biol. 2010;8:105.PubMed CentralPubMedView ArticleGoogle Scholar
- Greenberg AJ, Schedl P. GAGA factor isoforms have distinct but overlapping functions in vivo. Mol Cell Biol. 2001;21:8565–74.PubMed CentralPubMedView ArticleGoogle Scholar
- Bhat KM, Farkas G, Karch F, Gyurkovics H, Gausz J, Schedl P. The GAGA factor is required in the early Drosophila embryo not only for transcriptional regulation but also for nuclear division. Development. 1996;122:1113–24.PubMedGoogle Scholar
- Rorth P. A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. Proc Natl Acad Sci U S A. 1996;93:12418–22.PubMed CentralPubMedView ArticleGoogle Scholar
- Barges S, Mihaly J, Galloni M, Hagstrom K, Muller M, Shanower G, et al. The Fab-8 boundary defines the distal limit of the bithorax complex iab-7 domain and insulates iab-7 from initiation elements and a PRE in the adjacent iab-8 domain. Development. 2000;127:779–90.PubMedGoogle Scholar
- Karch F, Galloni M, Sipos L, Gausz J, Gyurkovics H, Schedl P. Mcp and Fab-7: molecular analysis of putative boundaries of cis-regulatory domains in the bithorax complex of Drosophila melanogaster. Nucleic Acids Res. 1994;22:3138–46.PubMed CentralPubMedView ArticleGoogle Scholar
- Iampietro C, Gummalla M, Mutero A, Karch F, Maeda RK. Initiator elements function to determine the activity state of BX-C enhancers. PLoS Genet. 2010;6:e1001260.PubMed CentralPubMedView ArticleGoogle Scholar
- Perez-Lluch S, Cuartero S, Azorin F, Espinas ML. Characterization of new regulatory elements within the Drosophila bithorax complex. Nucleic Acids Res. 2008;36:6926–33.PubMed CentralPubMedView ArticleGoogle Scholar
- Smith ST, Wickramasinghe P, Olson A, Loukinov D, Lin L, Deng J, et al. Genome wide ChIP-chip analyses reveal important roles for CTCF in Drosophila genome organization. Dev Biol. 2009;328:518–28.PubMedView ArticleGoogle Scholar
- Gruzdeva N, Kyrchanova O, Parshikov A, Kullyev A, Georgiev P. The Mcp element from the bithorax complex contains an insulator that is capable of pairwise interactions and can facilitate enhancer-promoter communication. Mol Cell Biol. 2005;25:3682–9.PubMed CentralPubMedView ArticleGoogle Scholar
- Hagstrom K, Muller M, Schedl P. Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex. Genes Dev. 1996;10:3202–15.PubMedView ArticleGoogle Scholar
- Zhou J, Barolo S, Szymanski P, Levine M. The Fab-7 element of the bithorax complex attenuates enhancer-promoter interactions in the Drosophila embryo. Genes Dev. 1996;10:3195–201.PubMedView ArticleGoogle Scholar
- Zhou J, Levine M. A novel cis-regulatory element, the PTS, mediates an anti-insulator activity in the Drosophila embryo. Cell. 1999;99:567–75.PubMedView ArticleGoogle Scholar
- Zhou J, Ashe H, Burks C, Levine M. Characterization of the transvection mediating region of the abdominal-B locus in Drosophila. Development. 1999;126:3057–65.PubMedGoogle Scholar
- Mihaly J, Barges S, Sipos L, Maeda R, Cleard F, Hogga I, et al. Dissecting the regulatory landscape of the Abd-B gene of the bithorax complex. Development. 2006;133:2983–93.PubMedView ArticleGoogle Scholar
- Holohan EE, Kwong C, Adryan B, Bartkuhn M, Herold M, Renkawitz R, et al. CTCF genomic binding sites in Drosophila and the organisation of the bithorax complex. PLoS Genet. 2007;3:e112.PubMed CentralPubMedView ArticleGoogle Scholar
- Maksimenko O, Kyrchanova O, Bonchuk A, Stakhov V, Parshikov A, Georgiev P. Highly conserved ENY2/Sus1 protein binds to Drosophila CTCF and is required for barrier activity. Epigenetics. 2014;9:1261–70.PubMedView ArticleGoogle Scholar
- Jeong S, Rokas A, Carroll SB. Regulation of body pigmentation by the Abdominal-B Hox protein and its gain and loss in Drosophila evolution. Cell. 2006;125:1387–99.PubMedView ArticleGoogle Scholar
- DiNardo S, Sher E, Heemskerk-Jongens J, Kassis JA, O'Farrell PH. Two-tiered regulation of spatially patterned engrailed gene expression during Drosophila embryogenesis. Nature. 1988;332:604–9.PubMed CentralPubMedView ArticleGoogle Scholar
- Duan H, Dai Q, Kavaler J, Bejarano F, Medranda G, Negre N, et al. Insensitive is a corepressor for Suppressor of Hairless and regulates Notch signalling during neural development. EMBO J. 2011;30:3120–33.PubMed CentralPubMedView ArticleGoogle Scholar
- Delorenzi M, Bienz M. Expression of Abdominal-B homeoproteins in Drosophila embryos. Development. 1990;108:323–9.PubMedGoogle Scholar
- Boulet AM, Lloyd A, Sakonju S. Molecular definition of the morphogenetic and regulatory functions and the cis-regulatory elements of the Drosophila Abd-B homeotic gene. Development. 1991;111:393–405.PubMedGoogle Scholar
- Holwerda SJ, de Laat W. CTCF: the protein, the binding partners, the binding sites and their chromatin loops. Philos Trans R Soc Lond B Biol Sci. 2013;368:20120369.PubMed CentralPubMedView ArticleGoogle Scholar
- Martinez SR, Miranda JL. CTCF terminal segments are unstructured. Protein Sci. 2010;19:1110–6.PubMed CentralPubMedView ArticleGoogle Scholar
- Maksimenko O, Bartkuhn M, Stakhov V, Herold M, Zolotarev N, Jox T, et al. Two new insulator proteins, Pita and ZIPIC, target CP190 to chromatin. Genome Res. 2015;25:89–99.PubMed CentralPubMedView ArticleGoogle Scholar
- Maeda RK, Karch F. The ABC of the BX-C: the bithorax complex explained. Development. 2006;133:1413–22.PubMedView ArticleGoogle Scholar
- Maeda RK, Karch F. Making connections: boundaries and insulators in Drosophila. Curr Opin Genet Dev. 2007;17:394–9.PubMedView ArticleGoogle Scholar
- Paro R. Imprinting a determined state into the chromatin of Drosophila. Trends Genet. 1990;6:416–21.PubMedView ArticleGoogle Scholar
- Pirrotta V. Polycomb silencing and the maintenance of stable chromatin states. Results Probl Cell Differ. 1999;25:205–28.PubMedView ArticleGoogle Scholar
- Ingham PW, Martinez-Arias A. The correct activation of Antennapedia and bithorax complex genes requires the fushi tarazu gene. Nature. 1986;324:592–7.PubMedView ArticleGoogle Scholar
- Muller J, Bienz M. Sharp anterior boundary of homeotic gene expression conferred by the fushi tarazu protein. Embo J. 1992;11:3653–61.PubMed CentralPubMedGoogle Scholar
- Zhang CC, Bienz M. Segmental determination in Drosophila conferred by hunchback (hb), a repressor of the homeotic gene Ultrabithorax (Ubx). Proc Natl Acad Sci U S A. 1992;89:7511–5.PubMed CentralPubMedView ArticleGoogle Scholar
- Qian S, Capovilla M, Pirrotta V. The bx region enhancer, a distant cis-control element of the Drosophila Ubx gene and its regulation by hunchback and other segmentation genes. Embo J. 1991;10:1415–25.PubMed CentralPubMedGoogle Scholar
- Mihaly J, Hogga I, Gausz J, Gyurkovics H, Karch F. In situ dissection of the Fab-7 region of the bithorax complex into a chromatin domain boundary and a Polycomb-response element. Development. 1997;124:1809–20.PubMedGoogle Scholar
- Karess RE, Rubin GM. Analysis of P transposable element functions in Drosophila. Cell. 1984;38:135–46.PubMedView ArticleGoogle Scholar