- Open Access
Travelling within the fetal gut: simple rules for an arduous journey
© Xu et al.; licensee BioMed Central 2014
- Received: 13 June 2014
- Accepted: 19 June 2014
- Published: 30 June 2014
The complex physiology of the gastrointestinal tract is regulated by intricate neural networks embedded within the gut wall. How neural crest cells colonize the intestine to form the enteric nervous system is of great interest to developmental biologists, but also highly relevant for understanding gastrointestinal disorders. A recent paper in BMC Biology addresses this issue with live imaging of gut explants from mouse embryos.
See research article: http://www.biomedcentral.com/1741-7007/12/23.
- Neural Crest Cell
- Enteric Nervous System
- Enteric Neuron
- Planar Cell Polarity
- Submucosal Plexus
The neuroectodermal networks of the gut remain in a state of flux long after the migratory front of NC cells has passed through a given segment of the intestine. This is clearly shown by experiments described by Young and colleagues which reveal that 24 to 48 hours after the arrival of the first NC cells in the developing small intestine, ENCCs continue to migrate extensively, although at this stage their trajectory is mostly circumferential with minimal rostrocaudal displacement. The predominantly radial migration of ‘older’ ENCCs could be imposed by changes in non-neuroectodermal tissues that accompany gut organogenesis, in particular the radial rearrangement of smooth muscle layers. The adoption by rostral ENCCs of a predominantly circumferential migration, which is ineffective in pushing cells caudally, is likely to explain why ENCCs must reach the end of the gut within a strict time frame: any delay in ENCC migration cannot be redressed later, and would make terminal gut regions neuron-free, thereby compromising the organ’s coordinated function. Nevertheless, the sustained ability of ENCCs to engage in local and radial migration means they can continue to add to and remodel enteric neural networks throughout embryogenesis and early life, when gut anatomy and physiology adjusts to feeding and the colonization of the intestine by microflora.
At present the only other well-studied example of ‘colonizing while migrating’ is the posterior lateral line primordium (pLLP), the placode-derived mechanosensory system that allows fish, and some amphibians, to detect even small changes in water movement over their bodies. Although some interesting parallels can be drawn between the migration of ENCCs and the migration of pLLP in zebrafish, whose transparent larvae make them particularly amenable to in vivo studies of cell migration, there are significant differences of cell behavior between the two systems. Thus, unlike the seemingly homogeneous population of ENCCs that ultimately are distributed throughout the gut wall, the pLLP remains as a well-defined and compact group of cells that maintains clear polarity along its length and deposits periodically from its trailing end rosette-like mechanosensory organ precursors []. In further contrast to the mammalian ENS, which continues to rearrange in an apparently unpredictable manner long after ENCCs have colonized the gut, pLLP maintains its organization and delivers an almost complete mechanosensory organ whose overall structure changes little following deposition. Interestingly, despite initially dispersing to form a diffuse ENS, ENCCs in zebrafish are initially organized into two compact columns that extend along either side of the gut [], perhaps suggesting a more stereotyped pattern of ENS development than seen in mice. The migratory behavior and hierarchical relationships of cells within the well-structured ENCC columns of zebrafish and their comparison to cells within pLLP and the mouse gut will certainly be the subject of future studies.
As ENCCs migrate and colonize the gut, a subset of them start to differentiate initially into neurons and later on into glial cells. In the mouse, enteric neurogenesis continues for several weeks after birth, highlighting the ability of newly born enteric neurons to be incorporated into fully functional neural circuits. The neural networks of the mammalian gut are extremely complex and the rules that govern their assembly and spatial organization are far from clear. One approach to clarifying the cellular and molecular mechanisms that regulate the formation of functional neural circuits in the ENS is to study the organization of nascent neurite modules in the embryonic gut. This approach has met some success recently when Sasselli et al. [] demonstrated that core components of the planar cell polarity (PCP) pathway are necessary for aligning neurites along the rostrocaudal axis of the gut. Mutations in this pathway resulted in a considerable degree of randomization of neurite orientation, which was associated with characteristic changes in the peristaltic activity of the intestine. Interestingly, despite the altered organization of neurites in PCP mutants, no obvious deficit in the colonization of the gut by NC cells was observed, arguing that migration of ENCCs is independent of neurite orientation. Consistent with this view, Young and colleagues argue that neurites in the gut follow a migratory path laid down by advancing ENCCs. In support of this view, placement of a group of ENCCs at the caudal end of an aneural gut segment from Ret mutant embryos resulted in rostral migration of ENCCs and the longitudinal arrangement of neurites that extended in the same direction. Intriguingly, this direction was maintained even after orally directed neurites met an advancing population of ENCCs that was placed at the opposite end. But if neurites simply follow the direction of ENCC migration, why do orally directed axons that encounter caudally migrating ENCCs continue to advance rostrally rather than randomizing their trajectory? Perhaps enteric neurites imbued with a migratory direction at an early critical stage of outgrowth by cognate ENCCs are unable to reroute upon encountering a caudally advancing group of NC cells. If this is indeed the case, it would be interesting to examine the cellular and molecular mechanisms that underpin the apparently irreversible commitment of axons of enteric neurons along the rostrocaudal and circumferential axes of the mammalian gut.
One of the most interesting aspects of the Young et al. paper is the suggestion that all the information required to drive key events in gut colonization by NCs and neural circuit development, such as the effective rostrocaudal advance of the migratory front and the organization and direction of neurite outgrowth, is intrinsic to ENCCs and largely independent of the polarity of gut mesenchyme. Does this indicate that the spatial distribution of neurotrophic factors produced by the gut wall have no effect on the migratory behavior of ENCCs and the growth cones of newly born enteric neurons? What about signaling molecules such as glial cell line-derived neurotrophic factor (GDNF), which is expressed by mesenchymal cells and, in addition to being critical for the survival and proliferation of ENS progenitors, functions as a guidance molecule for ENCCs in vitro [,]? Or members of the Wnt family, many of which are expressed in the embryonic gut and are critical in organizing neural networks in other parts of the nervous system? We suggest that there is no contradiction between the self-organizing ability of ENCCs and the proposed roles of these or other gut mesenchyme-derived factors. ENCCs and neurites could themselves contribute to the generation of local gradients of widely expressed mesenchymal factors (such as GDNF) that in turn direct their migration. The self-determined directional migration of cells through the local redistribution of extracellular cues has been demonstrated experimentally in pLLP []. In this case, gradients of the widely distributed chemokine Cxcl12a are generated across its length via polarized internalization of Cxcl12a by the receptor Cxcr7 at the trailing end and activation of Cxcr4 in the leading edge to propel the primordium. It would be of great interest to explore the potential role of the GDNF receptors Gfra1 and Ret in the generation of local GDNF gradients and the self-directed formation of a uniform ENS plexus.
Shedding light on the cellular and molecular mechanisms that control the colonization of the intestine by neural crest cells is of obvious medical importance. A number of conditions are thought to result from deficits in the migration and ultimately the colonization of the gut by neural crest cells and the normal development of the ENS. Foremost among them is Hirschsprung’s disease, a congenital neurodevelopmental deficit of the ENS, which occurs in 1:4,500 live births and is characterized by absence of enteric ganglia from the distal colon. In addition, a number of ill-defined conditions, termed collectively as functional gastrointestinal disorders, are thought to originate from subtle changes in the connectivity of enteric neurons. Therefore, understanding the mechanisms that regulate the migration of neural crest cells and how they may influence the connectivity of enteric neurons is critical for deciphering the pathogenesis of a broad range of congenital and acquired deficits of enteric neural activity and digestive function.
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