Induced metamorphosis in crustacean y-larvae: Towards a solution to a 100-year-old riddle
© Glenner et al; licensee BioMed Central Ltd. 2008
Received: 09 April 2008
Accepted: 20 May 2008
Published: 20 May 2008
The y-larva, a crustacean larval type first identified more than 100 years ago, has been found in marine plankton samples collected in the arctic, temperate and tropical regions of all oceans. The great species diversity found among y-larvae (we have identified more than 40 species at our study site alone) indicates that the adult organism may play a significant ecological role. However, despite intense efforts, the adult y-organism has never been identified, and nothing is therefore known about its biology.
We have successfully and repeatedly induced metamorphosis of y-larvae into a novel, highly reduced juvenile stage by applying the crustacean molting hormone 20-HE. The new stage is slug-like, unsegmented and lacks both limbs and almost all other traits normally characterizing arthropods, but it is capable of vigorous peristaltic motions.
From our observations on live and preserved material we conclude that adult Facetotecta are endoparasitic in still to be identified marine hosts and with a juvenile stage that represents a remarkable convergence to that seen in parasitic barnacles (Crustacea Cirripedia Rhizocephala). From the distribution and abundance of facetotectan y-larvae in the world's oceans we furthermore suggest that these parasites are widespread and could play an important role in the marine environment.
Additional file 1: Video 1 – Swimming y-cyprid. A y-cyprid swimming using its six pairs of thoracic appendages. The clip was recorded from a y-cyprid mounted under a cover slip, whence the body motions do not fully reflect natural swimming. When free in the water column, the y-cyprid swims almost continuously along a more or less straight line. (MPG 6 MB)
Additional file 2: Video 2 – Metamorphosing y-cyprid. Three phases in the metamorphosis of a single y-larva individual. The first phase shows the ypsigon about to escape, but with the posterior part of its body still located within the shell of the spent y-cyprid. The next phase shows the actual exit of the ypsigon from the y-cyprid. The third phase shows the free ypsigon shortly afterwards, when it has moved some distance away from the spent y-cyprid. (MPG 9 MB)
Additional file 3: Video 3 – Free ypsigon. An ypsigon that has just escaped from its y-cyprid. It exhibits moderate body movements (same specimen as shown in Figure 1B). (MPG 7 MB)
Additional file 4: Video 4 – Free ypsigon. A free ypsigon that has moved away from its y-cyprid and now exhibiting very vigorous body movements. (MPG 9 MB)
Additional file 5: Video 5 – Molting ypsigon. Ypsigon, 48 hours after escape from the y-cyprid. Now in the process of molting, while still exhibiting vigorous movements of the body (this is the same specimen as shown in Figures 2C and 2D). Note how the first ypsigon cuticle is clearly separated from the new one by a wide empty space. The internal structure of the ypsigon has not changed noticeably during the molt. (MPG 7 MB)
Like the preceding y-nauplius and y-cyprid stages, the ypsigon lacks a functional gut, but an elongated, dark mass of cells filled with fat globules and extending through most of the body may represent a vestige of this organ system (Figures 1B and 2C). Within the ypsigon body the muscles and compound eyes of the preceding y-cyprid stage are in a state of advanced degeneration (Figure 2A).
The ultrathin cuticle and the decomposing compound eyes are the only morphological indications of its arthropod affiliation (Figures 2A and 2B). According to the species tested, the resulting ypsigon could differ in morphology from being elongated to having a rather plump shape, but it always exhibited the same simplified structure (Figures 3, 4, 5). Ypsigons kept alive in culture for more than 24 hours passed through a molt that yielded another, second juvenile stage (Figures 2C and 2D). This second ypsigon stage had the same morphology as the first and continued to exhibit the same bending motions until they were preserved 48 hours after emerging from the y-cyprid (Additional file 5).
Within Arthropoda, the extreme morphological reduction seen in the new ypsigon stage is matched only by the early endoparasitic 'vermigon' stage recently discovered in certain parasitic barnacles, the Rhizocephala Kentrogonida [14, 15]. Both the vermigon and the ypsigon are formed by comparable metamorphic molts, in which the epidermis withdraws from the old cuticle, closes around all organs in the body and thereby forms the new slug-shaped stage. In addition, both the vermigon and the ypsigon exhibit vigorous body movements after their escape from the cypris, and they have virtually the same reduced morphology, being surrounded only by an exceedingly thin and very pliable cuticle and lacking segmentation, appendages and sensory organs. Metamorphic stages of rhizocephalans, similar to those emerging from naturally settled larvae [13, 16], have been produced when chemically inducing cyprids to metamorphose in vitro . By analogy with the rhizocephalan vermigon [15, 18] and our observation that the ypsigon does not change morphology at the first molt after escape, all evidence appears consistent with the ypsigon also being endoparasitic, eventually giving rise to a parasitic adult. This conclusion is also supported by comparison with the similarly apodous commiform larvae in the more distantly related entoniscid Isopoda [19, 20]. However, while rhizocephalans are incontestable members of the barnacles (Cirripedia), facetotectans diverge as a separate subclass at the base of the thecostracan phylogenetic tree and must represent a wholly separate evolution of extremely specialized parasites [5, 8, 9]. The evolution of very similar, slug-shaped endoparasitic stages (vermigon, ypsigon) in two different lineages (Rhizocephala, Facetotecta) is therefore a stunning example of convergent evolution. Analogy with the Rhizocephala also leads to the prediction that the adult facetotectan will turn out to have a highly simplified structure and this may help to explain why they have escaped observation. Future efforts will focus both on culturing the ypsigon in vitro to more advanced stages and screening of the local fauna to identify the host animals.
While we cannot yet identify the adult y-organism or its host, the new, highly aberrant and obviously endoparasitic stage in the life history of the Facetotecta takes us a major step towards the solution of an enigma that has puzzled zoologists for more than 100 years [2, 4, 10, 21–23]. It has been documented that parasites can significantly affect the structure of ecosystems, especially when they are parasitic castrators and/or are affecting host behavior . Our observation of more than 40 facetotectan 'species' at a single study site indicates that a hitherto unknown and diverse fauna of advanced endoparasites remain hidden and could play important roles in many marine habitats.
Field collection and handling of larvae
Plankton was caught several times daily in September and October of 2004 and 2005 from the pier of the Sesoko Laboratory, Tropical Biosphere Research Center, University of the Ryukyus, on the southeast coast of Sesoko Island (26°38'N 127°52'E). We used a hand-thrown net with a 62 μm mesh width, and complete daytime samples were sorted for larvae. Dusk and nighttime samples were prefiltered at 1000 μm to exclude larger organisms and make sorting easier. Samples were inspected and sorted under a dissection microscope with transmitted light. As in other thecostracan Crustacea, facetotectan species can have either feeding (planktotrophic) or non-feeding (lecithotrophic) nauplii, while cyprids never feed . From each plankton catch, we isolated the non-feeding y-nauplii into a 30 mm diameter plastic Petri dish with coarsely filtered (62 μm) seawater; any y-cyprids were isolated separately. The dishes were maintained at ambient room temperature (24–28°C). Those containing nauplius batch cultures were inspected at least three times daily and dead larvae were removed. When noted, stage-five nauplii, identified by the development of the pigmented compound eyes of the y-cyprid, were isolated singly into Petri dishes. After each stage-five nauplius had molted into a y-cyprid, the shed naupliar cuticle (exuvium) was individually mounted on a glass slide in glycerine jelly. This allowed us to document, which morphological type or 'species' it represented based on our extensive, unpublished records and collections of mounted larvae from this area. Occasionally y-cyprids appeared in the batch cultures between examinations, but apart from these and a few caught directly from the plankton, the great majority of all y-cyprids used in our induced metamorphosis trials (around 400) can thus be backtracked to a known naupliar type and be allocated to species, when a formal taxonomy for the Facetotecta is eventually established [3, 4, 6, 25].
For the induction of metamorphosis, single cyprids were introduced into standard plastic plates with 15 mm wells containing the test solutions and incubated at 24–28°C. A preliminary experiment was set up to test the effect of three different metamorphosis-inducing compounds on the y-cyprids. They were dissolved in Millipore-filtered seawater and used in concentrations tested previously on cyprids of parasitic barnacles (Rhizocephala) : 3-isobutyl-1-methylxanthine (IBMX at 4.5 μM), 20-hydroxyecdysone (20-HE at 208 μM, 20.8 μM, 2.08 μM, 1.04 μM and 208 nM), and juvenile hormone (JH at 37.5 nM, 375 nM and 3.75 μM). This screening showed that only 20-HE was able to induce the y-cyprids to metamorphose, and another preliminary trial established that the most efficient concentration was between 1.04 and 2.08 μM. All subsequent experiments were conducted within this range.
All y-cyprids tested were used within 24 hours after the molt from the nauplius stage five. Well plates were inspected several times daily using an Olympus inverted microscope. Stages of metamorphosis were photographed with an Olympus C5050 digital camera and digital videos obtained with a Sony HAD Power Head. The plastic well plates do not allow optimum conditions for microphotography. To obtain better micrographs and videos, selected specimens were temporarily transferred to glass slides with seawater and observed using a Nikon Microphot with up to 40× objectives. Digital images were optimized for contrast and color using Corel X3 suite. The only other manipulation was to touch up the background, not the specimen, in some scenes to remove extraneous particles.
Transmission electron microscopy
For TEM, selected stages of metamorphosing y-cyprids and free juveniles were fixed and stored in 2.5% glutaraldehyde in Millipore-filtered seawater buffered to pH 7.4 with 0.1 M sodium cacodylate. Following three rinses in the same buffer, specimens were postfixed in 1% OsO4, dehydrated with dimethoxypropane and embedded in TAAB 812 ('Epon') resin. Sections were mounted on slot grids, stained with uranyl-acetate and lead-citrate and inspected using a JEOL JEM-1011 transmission electron microscope fitted with a GATAN digital camera.
Funded by the Carlsberg Foundation and the Danish Natural Science Research Council (FNU; to JTH), the Villum Kann Rasmussen Foundation (to HG and JTH) and the Lake Biwa Museum (to MJG). We sincerely thank the Sesoko Station, University of the Ryukyus, for their hospitality, and Mr Y Nakano, Professor A Takemura and Mrs L Haukrogh for invaluable technical assistance. We dedicate this paper to the memory of the late Dr José Bresciani, who was the first to describe the y-cyprid stage and link it to y-nauplii.
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